Rapid and efficient extraction of PCR-ready genomic DNA from plant leaf samples
- Fast: PCR-ready DNA in about 8 minutes for most samples
- Simple: No bead-beating, freezing, or grinding of plant leaf material
- Increased Yields: No centrifugation or spin columns used which reduce yields
- Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
- Safe: Nontoxic reagets used throughout the procedure
The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). Most leafy plants are suitable for DNA extraction using the QuickExtract Plant Solution, including Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).
The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously, without grinding the sample, centrifugation, spin columns, or any toxic organic solvent. The procedure is fully compatible with robotic automation, provides a PCR-ready sample, and is reproducible (Fig. 3). Simply add the QuickExtract Plant solution to the sample and perform two sequential heating steps. A small aliquot of the sample is then used as a template for PCR or qPCR.
- High-throughput isolation of DNA from plant leaf samples for PCR-based analysis, e.g., GMO testing.
Figure 1. Overview of the QuickExtract™ Plant DNA extraction procedure.
|Figure 2. PCR products using QuickExtract™ Plant DNA Extraction Solution with different varieties of plant leaves. Forty cycles of RAPD were performed with DNA extracted from plant leaves using the QuickExtract Plant solution and the FailSafe™ PCR System. Lane M, 100-bp ladder; lane 1, pepper; lane 2, soybean; lane 3, spelt.
Figure 3. Reproducibility of PCR results with QuickExtract™ Plant DNA Extraction Solution from Arabidopsis thaliana leaves. Six individual punches of four Arabidopsis leaves were treated with QuickExtract Plant solution as described in the product information sheet. One microliter of the solution was used in a 25-µL PCR using the FailSafe™ PCR System and primers specific for the single-copy HSC70 chromosomal gene. Aliquots were analyzed on a 2% agarose gel and the DNA was visualized by staining with SYBR® Gold. Lanes 1-6, PCR products of extracted DNA samples;