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Remove all types of RNA and DNA with this robust, flexible nuclease.
- Non-specific: Digests all types of DNA and RNA including single- or double-stranded linear, circular, and supercoiled
- Flexible: Active within a broad range of conditions normally used in protein purification
- Customizable: Contact us for bulk or high concentration enzyme
OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides (Fig. 1). OmniCleave Endonuclease has the same substrate specificity and yields the same products as Benzonase®, an enzyme derived from Serratia marcescens.
Applications
- Removal of nucleic acids from cell lysates (reduction of viscosity) for improved handling and yield of protein preparations.
- Removal of trace contamination by nucleic acids in protein preparations.
- Removal of host DNA from phage preparations.
- Improved electrophoretic and chromatographic separation of proteins isolated from whole-cell lysates.
Unit Definition: One unit of OmniCleave Endonuclease converts 1.0 A260 (~60 µg) of sonicated salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in 50 mM Tris-HCl (pH 8.0 at 25°C) and 1 mM MgCl2.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5 at 25°C), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Quality Control: OmniCleave Endonuclease is free of detectable protease activity.
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Figure 1. Removal of nucleic acids from cell lysates using OmniCleave™ Endonuclease.Cell lysates were prepared with or without OmniCleave Endonuclease from E. coli cells expressing human lactate dehydrogenase B (LDH) and E. coli alkaline phosphatase (AP). Two microliters of each of the cell lysates were separated by electrophoresis on a 1% agarose gel and the nucleic acids detected by ethidium bromide staining. Lane M, 1-kb ladder. |
