A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity
- Synthesize DNA from ssDNA templates without degrading any single-stranded DNA
- Suitable for a variety of applications including random-primer labeling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3
Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´ to ;3´ and 3´ to ;5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355A and E357A).
Unit Definition: One unit of Exo-Minus Klenow DNA Polymerase (D355A, E357A) converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.
Quality Control: Exo-Minus Klenow DNA Polymerase (D355A, E357A) is free of detectable endo- and exonuclease and RNase activities.
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