Effectively digest large or small dsDNA and ssDNA into mononucleotides
- Digest unwanted dsDNA and ssDNA molecules including small ssDNA such as random hexamers
- Use in sensitive applications requiring reverse transcription where any contaminating DNA is unwanted
- Removal of genomic DNA from small-sample total RNA preparations for expression analysis (Fig. 1)
- Removal of small DNA oligonucleotides (e.g., random primers)
Baseline-ZERO™ DNase* digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I. Even the small DNA oligonucleotides that remain after treatment with bovine pancreatic DNase I are undetectable by gel electrophoresis following treatment with Baseline-ZERO DNase (Fig. 2). Removal of DNA from RNA preparations is particularly beneficial when RNA in a sample is amplified using a method that involves reverse transcription using random primers, since any contaminating DNA would also be a template for random-primed cDNA synthesis.
Figure 1. Real-time PCR of HeLa RNA preparations treated with various DNases. The lower the CT value (intersection of curves with the red line), the greater the amount of residual DNA not digested by the indicated DNase. Thus, Baseline-ZERO™ DNase removed all detectable DNA from the RNA sample. The TaqMan® probe assay amplified a 268-bp fragment of β-actin. Samples were run in duplicate.