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Now you can dilute BigDye 5:1 for diterminator sequencing! BetterBuffer enhances binding of primers and increases polymerase performance, especially with GC rich templates. This dilution / enhancement buffer is optimized for use with BigDye Terminator Cycle Sequencing with the 377, 3100, 3500, 3700, 3730. Mix 5 ul of BetterBuffer with 1 ul of BigDye Terminator mix. Add DNA and primers to a final volume of 15 ul. BetterBuffer: 10 x 0.3 mL tubes. Store at -20�? C.