When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pair reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody.
The Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectro- phoresis and immunoprecipitation.
- Specific properties of antigen & antibody.
- Antigen-antibody diffusion, interaction, and complex formation.
- Teaches Ouchterlony Double Diffusion and Radial Immunodiffusion.
- Application of antigen-antibody interaction in research laboratories.
Please refer to the Product Protocol for additional equipment needed.
Click here to download Product Protocol.