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Recombinant Protein Purification

$322.00

Molecular cloning is a commonly used technique for the generation of recombinant proteins.  The gene for a protein of interest is cloned into a vector, or plasmid, in frame with the...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
Recombinant Protein Purification For 6 groups of 4-5 or 24-30 students. BE-419 In stock or 2-3 weeks G Biosciences $322.00
PRODUCT INFORMATION

Molecular cloning is a commonly used technique for the generation of recombinant proteins.  The gene for a protein of interest is cloned into a vector, or plasmid, in frame with the gene for a protein tag.  These tags are used to purify expressed protein by established methodologies.  The most common tags in use consist of the six histidine motif (6XHis), purified on a nickel metal chelating column; the glutathione S-transferase (GST) tag, purified with a glutathione resin; and the calmodulin binding peptide (CBP) tag, isolated with calmodulin resin.

The Recombinant Protein Purification kit teaches students the basic principles of purifying recombinant proteins and has a hands-on lab activity that teaches students the purification of a tagged protein.  The recombinant protein supplied is a light emitting protein that allows for easy detection of the purified protein, using UV light.  Students will learn about protein tags and affinity chromatography.

Applications

This lab activity teaches the following:

  • Strategies used in the purification of recombinant proteins.
  • Purification of a recombinant protein.
  • Purification of recombinant tagged protein.

Please refer to the Product Protocol for additional equipment needed.

Click here to download Product Protocol.