Proteomics often involves the purification and identification of novel proteins from tissues. The strategy often employed is one using multiple and different protein purification techniques to fractionate proteins into specific fractions. This allows for easier identification and characterization of novel proteins.
The Purification of Proteins from Tissue kit is a comprehensive kit designed to teach students the principle of utilizing numerous purification techniques to identify novel proteins. Students will purify proteins from a mammalian tissue (mouse liver) and will use various fractionation and chromatography techniques to purify three target proteins from the mouse liver extract. The purification procedure will be closely monitored and easily visualized with gel electrophoresis. Students will then be able to assign specific characteristics to the isolated proteins, based on the purification techniques. This kit teaches protein fractionation, gel filtration and hydrophobic chromatography.
- Step-by-step approach to protein purification.
- Protein purification strategies.
- Perform salt fractionation, gel filtration and Hydrophobic chromatography.
Please refer to the Product Protocol for additional equipment needed.