The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilizing a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.
- Hands-on hydrophobic chromatography lab activity.
- The principle of hydrophobic chromatography.
- Immobilization of protein on hydrophobic columns.
- Factors influencing binding and elution of proteins on hydrophobic columns.
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