huge differences in abundance. These restrictions mean low abundance, critical proteins are often masked and are therefore hard to identify and purify. One key tool in resolving this issue is to utilize protein fractionation techniques.
The Protein Fractionation kit teaches common protein fractionation techniques used during protein purification, including acid and salt fractionation. The individual or combined use of acid and salt fractionations allows for the concentration and enrichment of proteins into defined fractions dependent on their precipitation at differing salt concentrations and/or pH. Students understand the importance of enrichment of target proteins in the scheme of purification of protein molecules. This lab activity involves preparation of a crude protein extract and fractionation of proteins by incremental changes in pH and salt concentration.
This lab activity teaches the following:
- Strategies in protein purification.
- Principles of protein fractionation.
- Protein fractionations by changing pH.
- Protein fractionation by increasing salt concentration.
Please refer to the Product Protocol for additional equipment needed.