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Protein Folding Study

$304.00

A proteins life cycle begins once its gene is turned on and its mRNA is transcribed and translated to produce the polypeptide strand. The correct folding of a protein is the...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
Protein Folding Study For 6 groups of 4-5 or 24-30 students. BE-411 In stock or 2-3 weeks G Biosciences $304.00
PRODUCT INFORMATION

A proteins life cycle begins once its gene is turned on and its mRNA is transcribed and translated to produce the polypeptide strand. The correct folding of a protein is the first crucial stage of the life cycle of a protein. Correct folding is essential for a protein to be functional, whereas incorrect folding can have severe detrimental effects.  For example, several misfolded protein diseases include bovine spongiform encephalopathy (BSE) and its human equivalent Creutzfeld-Jakob disease (CJD), Alzheimer’s disease, Parkinson’s disease, type II (non-insulin dependent) diabetes and some types of cancer. The symptoms of Mad Cow Diseases (BSE) and Alzheimer’s are a result of misfolded proteins aggregating and forming insoluble protein deposits in the brain.

Protein unfolding and folding, a reversible process, is analyzed using a light emitting protein specifically prepared for this study and a protein electrophoresis technique. The kit allows students to see when a light emitting protein is denatured  (unfolded) as it loses its property to emit light.  After electrophoresis, the protein in the gel is subjected to a renaturation and folding treatment, which returns the protein to its natural configuration resulting in a return of  the light emitting properties.

Applications

This lab activity teaches the following:

  • The principles of protein denaturation and refolding.
  • The importance of correct folding for protein activity.
Please refer to the Product Protocol for additional equipment needed.

Click here to download Product Protocol.