This lab activity has two objectives, on one hand it is designed to deepen the understanding of protein molecules. On the other hand, students also learn the potential of electrophoresis in protein analysis.
Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilizing protein electrophoresis. Complex mixture of protein samples and characterized pure protein samples, in conjunction with electrophoresis, are utilized to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein sub-units.
This lab activity teaches the following:
- Potential of electrophoresis in protein research.
- Use of electrophoresis for protein characterization.
- Parameters affecting protein electrophoresis analysis.
- Analysis of proteins structure by electrophoresis.
- Role of denaturing and reducing agents in protein electrophoresis.
- Protein degradation, breakdown, & role of protease.
Please refer to the Product Protocol for additional equipment needed.
Click here to download Product Protocol.