Students will explore the principles of bacterial transformation and will learn how to make bacteria susceptible, or competent, for transformation. They will also transform bacteria with DNA that confers antibiotic resistance and encodes for a light emitting protein. The results of the transformation can be easily visualized under UV light as glowing bacteria.
- Preparation of Competent cells.
- Bacterial transformation.
- Antibiotic selection of transformed bacteria.
Please refer to the Product Protocol for additional equipment needed.