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The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
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The blots are ready to be blocked and probed with the antibodies of choice.
Features
Blot lane order:
- Protein marker
- Green Algae Lysate
- Yeast Lysate
- Barley Lysate
- Sweet Corn Lysate
- Rye Grass Lysate
- Oat Lysate
- Wheat Lysate
- Soy Lysate
- Mung Lysate
- Sunflower Lysate
- Cotton Lysate
- Beet Lysate
- Tomato Lysate
- Spinach Lysate
Please refer to the Product Protocol for additional equipment needed.
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