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BAC-Optimized Replicator-v2.0 ElectroCOMPETENT CELLS,12 rxns (DUOs)

$446.00

BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells Cells that enable the most challenging library construction and screening. Highest available transformation efficiencies for library construction and screening. Optimized for high yields...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
BAC-Optimized Replicator-v2.0 ElectroCOMPETENT CELLS,12 rxns (DUOs) EACH 60210-1 In stock or 2-3 weeks Lucigen $446.00
PRODUCT INFORMATION

BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.

Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight®Cloning Kits.

E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC or pKanR DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G BAC-Optimized Electrocompetent

>1 × 1010

YES

YES

YES
without IPTG induction

BAC-Optimized Replicator v2.0 Electrocompetent

>1 × 1010

YES

YES

YES
IPTG induction required