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Table 1. E. cloni Competent Cells for Cloning and Library Construction
|
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G SUPREME Electrocompetent |
>4 × 1010 |
YES |
YES |
YES |
|
10G ELITE Electrocompetent |
>2 × 1010 |
YES |
NO |
YES |
|
10G CLASSIC Electrocompetent |
>5 × 109 |
YES |
NO |
YES |
|
10GF´ ELITE Electrocompetent |
>2 × 1010 |
YES |
NO |
YES |
Efficiencies
- E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
- E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
- E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
