- Reliable transcription
T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
Unit Concentration | 50,000 U/mL |
Purity (SDS-PAGE) | >99% |
SS Exonuclease | 500 U <0.1% released |
DS Exonuclease | 500 U <0.1% released |
Endonuclease | 500 U <10% converted |
RNAse Contamination | 500 U = none detected |
E. coli 16S rDNA Contamination | 500 U <10 copies |
Storage | -20°C |