- Excellent strand displacement, fidelity and speed.
phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.
Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
|Unit Concentration||10,000 U/mL|
|E. coli 16S rDNA Contamination||100 U <10 copies|
|Endonuclease||100 U <10% converted|