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- Excellent strand displacement, fidelity and speed.
phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.
Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
| Unit Concentration | 10,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | Functional |
| E. coli 16S rDNA Contamination | 100 U <10 copies |
| Endonuclease | 100 U <10% converted |
| Storage | -20&°C |
