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![ProteaseArrestâ„¢ for Plant [100X]](http://astralscientific.com.au/cdn/shop/products/plant@2x.jpg?v=1631166402)
**Click Here to Download Protease Inhibitor Cocktail Selection Guide**
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, 1,10-Phenathroline and PMSF that inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases. This protease inhibitor cocktail is specifically designed for the inhibition of proteases in plant cell and tissue lysates.Â
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Sample Type & key features |
Inhibition Specificity |
1X Composition |
Concentration & Available Sizes |
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For plant tissue and cell extracts EDTA is supplied in a separate vial |
Plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases |
668µM AEBSF 3µM Bestatin 14µM E-64 5.25µM Leupeptin 1µM Pepstatin A 2mM 1,10-Phenathroline 1mM PMSF |
100X DMSO solution 1ml, 5ml |
Features
- Plant specific protease inhibitor cocktail
- 100X concentrated, ready-to-use solution
- No proprietary ingredients, fully disclosed formulation of seven broad range protease inhibitors, including AEBSF, Bestatin, E-64, Leupeptin, Pepstain A, 1,10-Phenathroline and PMSF
Applications
- Inhibition of protease activity in protein preparations of plant lysates.
- Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays, and other applications.
ProteaseArrest Family
Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species. In addition to Bacteria, species-specific cocktails are available for Bacterial, Yeast, Fungus and Mammalian samples. Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilised Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. The TCM ProteaseARREST™ protease inhibitor cocktail is a broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases. Designed for the protection of proteins secreted in to the culture media.
