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- Reliable transcription
T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
| Unit Concentration | 50,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | 500 U <0.1% released |
| DS Exonuclease | 500 U <0.1% released |
| Endonuclease | 500 U <10% converted |
| RNAse Contamination | 500 U = none detected |
| E. coli 16S rDNA Contamination | 500 U <10 copies |
| Storage | -20°C |
