Exo-Minus Klenow DNA Poly (D355A, E357A), 1,000 U @ 10 U/uL

A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity

• Synthesize DNA from ssDNA templates without degrading any single-stranded DNA
• Suitable for a variety of applications including random-primer labeling of DNA,¹second-strand cDNA synthesis,^2sStrand-displacement amplification³

Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´ to ;3´ and 3´ to ;5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355A and E357A).

Unit Definition: One unit of Exo-Minus Klenow DNA Polymerase (D355A, E357A) converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.

Quality Control: Exo-Minus Klenow DNA Polymerase (D355A, E357A) is free of detectable endo- and exonuclease and RNase activities.

References

1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.
2. Sanbrook, J. et al. (1989) In: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. v.2, p. 8.14.
3. Walter, N.G. (1995) J. Mol. Biol. 254, 856.