Baseline-ZERO-DNase, 5,000 U

Effectively digest large or small dsDNA and ssDNA into mononucleotides

• Digest unwanted dsDNA and ssDNA molecules including small ssDNA such as random hexamers
• Use in sensitive applications requiring reverse transcription where any contaminating DNA is unwanted

Applications

• Removal of genomic DNA from small-sample total RNA preparations for expression analysis (Fig. 1)
• Removal of small DNA oligonucleotides (e.g., random primers)

Baseline-ZERO™ DNase* digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I. Even the small DNA oligonucleotides that remain after treatment with bovine pancreatic DNase I are undetectable by gel electrophoresis following treatment with Baseline-ZERO DNase (Fig. 2). Removal of DNA from RNA preparations is particularly beneficial when RNA in a sample is amplified using a method that involves reverse transcription using random primers, since any contaminating DNA would also be a template for random-primed cDNA synthesis.

Figure 1. Real-time PCR of HeLa RNA preparations treated with various DNases. The lower the C_T value (intersection of curves with the red line), the greater the amount of residual DNA not digested by the indicated DNase. Thus, Baseline-ZERO™ DNase removed all detectable DNA from the RNA sample. The TaqMan® probe assay amplified a 268-bp fragment of β-actin. Samples were run in duplicate.