• Cells that enable the most challenging library construction and screening.
• Highest available transformation efficiencies for library construction and screening.
• Optimized for high yields of DNA.

Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 10¹⁰ cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight®Cloning Kits.

E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 10⁷ cfu/µg 150 kb BAC DNA; > 1 × 10¹⁰ cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning