NxSeq-UltraLow DNA Library Kit, 12 Reactions

Lowest input, highest efficiency, Illumina-compatible DNA fragment library prep kit

• High Quality Data: High efficiency adapter ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions

• Sensitive: Construct DNA fragment libraries from as little as 50 pg to as much 75 ng of sheared/fragmented DNA

• Minimal Bias: Robust, uniform PCR amplification improves coverage uniformity when working with low input amounts of genomic DNA

• Flexible:Extensively tested in de novo whole genome sequencing or resequencing, but compatible with other applications such as exome-seq, ChIP-seq and FFPE DNA samples.

• Fast: 3 hour protocol gets your samples on the sequencer quicker

• High Value: Cost-effective library and indexing kits which produce excellent sequencing results

 The NxSeq® UltraLow DNA Library Kit and NxSeq Single Indexing Kits are only compatible with Illumina sequencers.

The NxSeq UltraLow DNA Library Kit, 12 Reactions contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG), 2X PCR Master Mix (MM) and Elution Buffer (EB). At least one NxSeq Single Indexing Kit is required to complete library prep and must be purchased separately.

Each NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. Each NxSeq® Single Index equals the TruSeq® LT Index with the same number.