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Plasmid Isolation (Alkaline Lysis)

$375.00

Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques.  The purification of DNA is important for genetic research as it provides a source of transferable...

NAME SIZE ID AVAILABLITY VENDOR PRICE EX GST $35 FREIGHT FEE PER ORDER
Plasmid Isolation (Alkaline Lysis) For 6 groups of 4-5 or 24-30 students. BE-310 Enquire for stock availability G Biosciences $375.00
PRODUCT INFORMATION
Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques.  The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA.
 
One common technique for plasmid purification is the alkaline lysis method, which breaks open bacteria with an alkaline solution, proteins are removed by precipitation and the plasmid DNA is recovered with alcohol precipitation.
 
Students purify bacterial plasmids from a liquid culture using this alkaline lysis method.
 
Applications
  •  Teaches alkaline lysis plasmid purification.
  • Teaches ethanol precipitation.

Please refer to the Product Protocol for additional equipment needed.

Click here to download Product Protocol. 

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