OneQuantâ„¢ DTT [0.5M]

DTT is a common reducing agent used for the cleavage of disulfide bonds. OneQuant™ DTT are single aliquots of DTT that eliminate the need for weighing, preventing loss of reagent and saving time.  

Description: OneQuantâ„¢ DTT

Size: 7.7mg/vial; 5 strips of 8 vials each

Storage conditions: Shipped at ambient temperature. Upon receipt, store product at ‐20°C.  

Information

• Synonyms: 1, 4‐Dithiothreitol; DTT; Cleland’s Reagent; 1,4‐Dimercapto‐2,3‐ Butanediol

• Molecular Weight: 154.24

• Formula: C2H10O2S2

• Appearance: white solid  

Protocol

1. Cut an individual vial from the unused vials. Open the vial and add 90μl distilled water or buffer, to generate a 500mM solution.
2. Gently mix with a pipette tip, or with gentle vortexing. The DTT normally dissolves within 30 seconds.
3. Use the DTT solution immediately and discard any unused DTT solution.

 

Click here to download product protocol