NxGen phi29 DNA Polymerase, 10U/uL, 10000 units

phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

The enzyme storage buffer consists of 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C and the 10X phi29 DNA Polymerase Buffer is composed of 500 mM Tris-HCl, 100 mM (NH₄)₂SO₄, 40 mM Dithiothreitol, 100 mM MgCl₂, pH 7.5 @ 25°C.