EconoTaq-PLUS 2X Master Mix, 500 rxns

• Outstanding performance and value are perfect for routine PCR.
• Can be cycled up to 98°C to amplify the most challenging GC-rich templates others cannot.
• Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer.
• Non-proofreading polymerase
• EconoTaq PLUS GREEN contains tracking dyes for gel electrophoresis

Performance, Convenience, Reliability, and Value for Routine PCR.

• More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes (Figure 1). High density reaction buffer enables direct gel loading following PCR.
• Excellent results in routine PCR. EconoTaq PLUS GREEN & EconoTaq PLUS contain high purity, high activity EconoTaq DNA Polymerase for reliable amplification of templates up to 5 kb (Figure 1).
• Amplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98°C to amplify challenging GC-rich templates others cannot (Figure 2).
• Convenient tracking dyes in EconoTaq PLUS GREEN. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp. See Figure 3. If desired, the dyes are easily removed by standard DNA purification products or ethanol precipitation. For fluorescence or absorbance detection assays, EconoTaq PLUS 2X Master Mix offers the same great performance without the dyes.
• Flexible & easy to use. EconoTaq PLUS GREEN and EconoTaq PLUS are packaged in 50 reaction vials that can be stored in the refrigerator (+4ºC) for up to 3 months. They are stable for at least 10 freeze-thaw cycles.

Figure 1. EconoTaq PLUS GREEN Master Mix vs. GoTaq® Green master mix (Promega) in PCR. PCR was performed according to the manufacturer’s recommendations using primers specific for the indicated genes. Panel A, 0.7 kb prokaryotic single-stranded DNA binding protein (genomic DNA); Panel B, 2.7 kb prokaryotic DNA polymerase A (genomic DNA); Panel C, 4.5 kb gene (plasmid DNA).