E. cloni® 5-alpha Chemically Competent Cells
- Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.).
- Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Table 1. E. cloni Competent Cells for Cloning
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
5-alpha Chemically Competent |
>1 × 108 cfu/µg DNA |
NO |
NO |
YES |