General cloning strain

• Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.
• Achieve high efficiencies with convenient chemically competent format: >5 × 10⁸ cfu/µg pUC19 DNA.
• Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations.

Applications

• Routine cloning of DNA up to 200 kb.

The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).

Benefits

• High transformation efficiency with clones of all sizes, including BAC clones (Table 1).
• lacZΔM15 for blue/white screening of recombinants.
• Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
• Endonuclease minus (endA1) to ensure high yields of DNA.
• Recombination minus (recA1) for greater stability of large cloned inserts.

Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.

Genotype

F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ^- rpsL (Str^R) nupG

TransforMax EC100 Electrocompetent E. coli

• Transformation efficiency of >1 × 10¹⁰ cfu/µg of pUC19.

TransforMax EC100 Chemically Competent E. coli

• Transformation efficiency of >5 × 10⁸ cfu/µg of pUC19.