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14 Frequently asked questions, 2Protocols on CHAPS you should know

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Chaps Structure


A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS have been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel. A commonly used isoelectric focusing sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT) and 40 mM Tris. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. It can be used as a non-toxic stabilizing agent for growth factors.

Molecular Formula: C32H58N2O7S
Molecular Weight: 614.9
Density: 1.01g/mL at 20 °C
CAS #: 75621-03-3
Synonyms: 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N, N-Dimethyl-N-(3-sulfopropyl)-3-[(3a, 5b, 7a, 12a)-3, 7, 12-trihydroxy-24-oxocholan-24-yl] amino

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