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End-It-DNA End-Repair Kit, 20 rxns

$231.00

Convert DNA with damaged or incompatible 5´- and/or 3´-protruding ends into ready-to-ligate, 5´-phosphorylated, blunt-ended DNA. Complete conversion of DNA into blunt-ended, phosphorylated DNA Repair sheared, nebulized or restriction enzyme-digested genomic DNA...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
End-It-DNA End-Repair Kit, 20 rxns EACH ER0720 In stock or 2-3 weeks Lucigen $231.00
PRODUCT INFORMATION

Convert DNA with damaged or incompatible 5´- and/or 3´-protruding ends into ready-to-ligate, 5´-phosphorylated, blunt-ended DNA.

  • Complete conversion of DNA into blunt-ended, phosphorylated DNA
  • Repair sheared, nebulized or restriction enzyme-digested genomic DNA
  • Prepare templates for NGS (next-generation sequencing) or shotgun library preparation

Applications

The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit.

The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).

Figure 1. DNA fragments containing any type of ends are rapidly and efficiently converted to 5´-phosphorylated, blunt-ended DNA using the End-It™ DNA End-Repair Kit.The end-repaired DNA is ready for blunt-end ligation using, for example Epicentre's Fast-Link™ DNA Ligation Kit.