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ALLin Taq DNA Polymerase, 5 u/µl, 2500u

$1,511.00

Applications Routine PCR up to 6 kb PCR of GC rich templates Colony PCR, Fast PCR, TA cloning Benefits Higher yields under standard and fast cycling Increased success in amplification of...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
ALLin Taq DNA Polymerase, 5 u/µl, 2500u Default Title PCE0105 In stock or 2-3 weeks highQu $1,511.00
PRODUCT INFORMATION

Applications

  • Routine PCR up to 6 kb
  • PCR of GC rich templates
  • Colony PCR, Fast PCR, TA cloning


Benefits

  • Higher yields under standard and fast cycling
  • Increased success in amplification of longer (6 kb) and GC rich templates
  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTP concentrations

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (a number of correct nucleotides incorporated before the first error). ALLin™ Taq DNA Polymerase produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Taq Mastermix, 2X is available.

 

Important Notes
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
  • The longer the amplicon, the longer the extension time: 15 sec/kb - <6kb and 1 sec/kb - <1kb. Use >90 sec extension for multiplexing
  • Run an annealing temperature gradient from 55°C to 65°C to choose the best specificity conditions.
  • Do not use fast cycling for multiplexing.
 
Prepare a 50 µl PCR reaction

 

Rev. & For. Primers variable, up to 0.4 µM final each (2 µl of 10 µM each)
cDNA Template  or
gDNA Template
<100 ng   or
5 - 500 ng
5X ALLin™ PCR Buffer 10 μl
Water to 49 μl
ALLin™ Taq DNA Polymerase, 5u/μl
0.25 - 1 µl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

 

Initial denaturation 1 cycle: 95°C - 1 min
Denaturation 40 cycles: 95°C - 15 sec
Annealing 40 cycles: 55-65°C – 15 sec
Extension 40 cycles: 72°C – 1- 90 sec (15 sec/kb)

 

  • After the PCR store probes on ice shortly, store at -20°C for long term.
Cat. Size Components Composition

PCE0101

500 u

500 u - ALLin™ Taq DNA Polymerase, 5 u/µl
4 x 1 ml - 5X ALLin™ PCR Buffer
Enzyme in storage buffer.
1X ALLin™ PCR Buffer contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers.

PCE0105

2500 u

5 x 500 u - ALLin™ Taq DNA Polymerase, 5 u/µl
20 x 1 ml - 5X ALLin™ PCR Buffer
Enzyme in storage buffer.
1X ALLin™ PCR Buffer contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers.

Storage

   In the dark at -20°C.