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ALLin Red Taq Mastermix, 2X, 200 r of 50 µl

$209.00

Applications Routine PCR up to 6 kb with a direct gel loading option PCR of GC rich templates Colony PCR, Fast PCR, TA cloning Benefits Premixed with the dye and density...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
ALLin Red Taq Mastermix, 2X, 200 r of 50 µl Default Title PCM0201 In stock or 2-3 weeks highQu $209.00
PRODUCT INFORMATION

Applications

  • Routine PCR up to 6 kb with a direct gel loading option
  • PCR of GC rich templates
  • Colony PCR, Fast PCR, TA cloning

Benefits

  • Premixed with the dye and density reagent for direct loading on the gels
  • Higher yields under standard and fast cycling
  • Success with longer (6 kb) and GC rich templates

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.

The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors.

ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR.

In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.

The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.

 

mportant Notes
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.
  • The longer the amplicon, the longer the extension time: 15 sec/kb - <6kb and 1 sec/kb - <1kb. Use >90 sec extension for multiplexing
  • Run an annealing temperature gradient from 55°C to 65°C to choose the best specificity conditions.
  • Do not use fast cycling for multiplexing.
 
Prepare a 50 µl PCR reaction

 

Rev. & For. Primers variable, up to 0.4 µM final each (2 µl of 10 µM each)
cDNA Template  or
gDNA Template
<100 ng  or
5 - 500 ng
PCR Water to 25 μl
ALLin™ Red Taq Mastermix, 2X 25 µl

 

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

 

Initial denaturation 1 cycle: 95°C - 1 min
Denaturation 40 cycles: 95°C - 15 sec
Annealing 40 cycles: 55-65°C - 15 sec
Extension 40 cycles: 72°C – 1 - 90 sec (15 sec/kb)

 

  • Load probes on the agarose gel. The red loading dye is included in the mastermix.

  • ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.

  • Store probes on ice shortly, store at -20°C for long term.

Cat.

Size

Components

Composition

PCM0201 200 r of
50 µl
5 x 1 ml - ALLin™ Red Taq Mastermix, 2X
5 x 1 ml - PCR Water
1X mastermix contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers, red electrophoresis tracking dye and density reagents for gel loading
PCM0205 1000 r of
50 µl
25 x 1 ml - ALLin™ Red Taq Mastermix, 2X
25 x 1 ml - PCR Water
1X mastermix contains 0.25 mM dNTPs, 3 mM MgCl2, enhancers, stabilizers, red electrophoresis tracking dye and density reagents for gel loading


Storage


  In the dark at -20°C.