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StainIN GREEN Nucleic Acid Stain, 20000X, 1ml

$206.00

Applications Staining of NA in agarose and polyacrylamide gels during electrophoresis for ssDNA, dsDNA and RNA visualization and gel documentation Blue LED detection, excellent for cloning applications UV detection Benefits Much...

NAME SIZE ID AVAILABLITY VENDOR Your PRICE ORDER
StainIN GREEN Nucleic Acid Stain, 20000X, 1ml Default Title NAS0201 In stock or 2-3 weeks highQu $206.00
PRODUCT INFORMATION

Applications

  • Staining of NA in agarose and polyacrylamide gels during electrophoresis for ssDNA, dsDNA and RNA visualization and gel documentation
  • Blue LED detection, excellent for cloning applications
  • UV detection


Benefits

  • Much more safe alternative to ethidium bromide, more economical alternative to competing green dyes
  • Unique – has two emission peaks, colors DNA in green, RNA in red
  • Highly sensitive NA detection - up to 4x more sensitive than EtBr
  • Time saving – in gel stain, no post- run staining, no destaining

 

StainIN™ GREEN Nucleic Acid Stain is a significantly safer alternative to ethidium bromide.

It is same easy to use, 4X as sensitive (under Blue LED) and much more secure. More economical as other green dyes, this stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide.

It is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels. It binds to both dsDNA, ssDNA and RNA and emits green fluorescence when bound to DNA and red fluorescence when bound to RNA detectable under the UV or Blue light and documented with same filters as similar green dyes.

StainIN™ GREEN is ideal for DNA extraction from gels for cloning. Smaller than ethidium bromide carcinogenicity has been proved by Ames-test. Mammalian cell mutagenicity tests, both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results.

Use highQu ready to use DNA ladders for electrophoresis. Use StainIN™ RED Nucleic Acid Stain in case you stay with ethidium bromide filters for photography.

 

Protocol for agarose gel electrophoresis

1. Wear gloves when working with all NA stains, buffers and gels.
2. Prepare the agarose gel solution like recommended by supplier.
3. Cool down the agarose after boiling to a hand-friendly temperature.
4. Add 5µl of StainIN™ GREEN solution per 100 ml of the gel right before casting the gel.
5. Mix the gel solution very gently to distribute the dye but not to produce air bubbles. Pour the gel, insert the combs.
6. Prepare the required volume of 1X TAE or 1X TBE buffer to be used in the electrophoresis tank.
7. Add 2,5 - 3 µl of StainIN™ GREEN solution per 100 ml of the 1X electrophoresis running buffer.
8. Add both gel and the buffer into the electrophoresis tank and run electrophoresis like usual.
9. Visualize NA under the Blue light or UV (~500-650 nm).

  • Destaining is not needed, but it might help to reduce the background; post-run staining is not recommended.
  • Use only Blue light if you intend to clone the DNA.
  • Use SYBRGreen filters for gel photography.
  • After a few runs refresh the electrophoresis buffer in electrophoresis tank prepared as in step 7.
  • If you reuse the gel, add at least half a portion of the stain each time after boiling and cooling the gel solution down (like in step 4).
 

Protocol for polyacrylamide gel electrophoresis

1. Wear gloves when working with all NA stains, buffers and gels.
2. Prepare the native or denaturing PAA gel solution like recommended by supplier.
3. Add TEMED and APS and proceed to the next step immediately.
4. Add 5µl of StainIN™ GREEN solution per 100 ml of the gel right before casting the gel.
5. Mix the gel solution very gently to distribute the dye but not to produce air bubbles. Pour the gel, insert the combs.
6. Prepare the required volume of 1X TBE buffer to be used in the electrophoresis tank.
7. Add 2,5 - 3 µl of StainIN™ GREEN solution per 100 ml of the 1X electrophoresis running buffer.
8. Add both gel and the buffer into the electrophoresis tank and run electrophoresis like usual.
9. Visualize NA under the Blue light or UV (~500-650 nm).

  • Destaining is not needed, but it might help to reduce the background; post-run staining is not recommended.
  • Use only Blue light if you intend to clone the DNA.
  • Use SYBRGreen filters for gel photography.
  • After a few runs refresh the electrophoresis buffer in electrophoresis tank prepared as in step 7.
CAT. SIZE COMPONENTS COMPOSITION

NAS0201

1 ml

1ml - StainIN™ GREEN Nucleic Acid Stain Aqueous solution of green DNA and RNA stain. Provided as 20000 X solution to be used at 1 X concentration in agarose or polyacrylamide gels and at 0,5 X concentration in electrophoresis buffers.
Storage and stability:   
Store in the dark at +4°C for at least two years.

DISPOSAL: Used dye solutions or melted gels shall be run through approved filters. If the absence of residual fluorescense is confirmed, the liquids can be disposed with plenty of water down the drain. Consult your safety office to match your local regulations, as they vary and change.