{"title":"Cross-Linking and Modification","description":"\u003cp\u003eCross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulfhydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules.  functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls,  carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents. Cross-linking agents are used to study protein structure and function, to anchor proteins to solid  supports, preparation of immunogens, immunotoxins, and other conjugated protein reagents. Astral Scientific offers a variety of Double Do™ cross-linking agents, reaction specific buffers, and accessories for performing and facilitating crosslinking applications. These\u003ca href=\"http:\/\/www.gbiosciences.com\/ResearchProducts\/Protein-Research\/Cross-Linking-and-Modification\/Accessories.aspx\"\u003e accessory tools\u003c\/a\u003e simplify cross-linking reactions and will enable researchers to achieve highly efficient cross-linking reactions with minimal effort in reaction optimization, set-up, and procedure development\u003c\/p\u003e\n\u003cp\u003eWe also offer a variety of amino acid side chain modifiers to block side chain interactions, change the charge or modify them to be more favourable for other reactions. Many \u003ca href=\"http:\/\/www.gbiosciences.com\/ResearchProducts\/Protein-Research\/Cross-Linking-and-Modification\/Denaturants.aspx\"\u003edenaturants, or chaotropes\u003c\/a\u003e, are available and can disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. 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Buffer","offer_id":18640907370563,"sku":"786-234","price":661.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/chemicals_12_4c114c51-dc7b-410f-8569-45b79e18767d.png?v=1745802081"},{"product_id":"786-238","title":"TCM Protease Arrestâ„¢","description":"\u003cp align=\"left\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/files\/AS_Protease_inhibitors_GBioscience_v1.pdf?v=1631168776\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/files\/AS_Protease_inhibitors_GBioscience_v1.pdf?v=1631168776\"\u003e\u003cstrong\u003e**Click Here to Download Protease Inhibitor Cocktail Selection Guide**\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003eA broad range, 200X concentrated, sterile filtered, ready-to-use protease inhibitor cocktail for the protection of proteins secreted into tissue culture media. TCM ProteaseArrestâ„¢ features optimized concentrations of Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A that inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases  trypsin-like and acid proteases. The inhibitor cocktail is designed to protect secreted proteins during cell culture for up to 48 hours.\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"0\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Type \u0026amp; key features\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInhibition Specificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e1X Composition\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration \u0026amp; Available Sizes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFor protection of secreted proteins in tissue culture media.\u003c\/p\u003e\n\u003cp\u003eSterile filtered\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eA wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.6µM Aprotinin\u003c\/p\u003e\n\u003cp\u003e58µM Bestatin\u003c\/p\u003e\n\u003cp\u003e28µM E-64\u003c\/p\u003e\n\u003cp\u003e10.5µM Leupeptin\u003c\/p\u003e\n\u003cp\u003e2.1µM Pepstatin A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e200X Sterile filtered DMSO solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNo proprietary ingredients, fully disclosed formulation of five broad range protease inhibitors, including Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A\u003c\/li\u003e\n\u003cli\u003eBroad range protease inhibitor cocktail\u003c\/li\u003e\n\u003cli\u003eStable for up to 48 hours in cell culture media\u003c\/li\u003e\n\u003cli\u003eSterile filtered\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProtect secreted proteins from proteolytic degradation during cell culture \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eProteaseArrest Family\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003eOur\u003cspan\u003e \u003c\/span\u003eProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003eis a highly efficient, liquid format general protease inhibitor cocktail that offers \u0026gt;95% protease inhibition. The ProteaseArrestâ„¢ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species.  In addition to Bacteria, species-specific cocktails are available for\u003cspan\u003e \u003c\/span\u003eBacterial, Plant, Yeast,\u003cspan\u003e \u003c\/span\u003eFungus and\u003cspan\u003e \u003c\/span\u003eMammalian\u003cspan\u003e \u003c\/span\u003esamples. Our\u003cspan\u003e \u003c\/span\u003eRecom ProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003eis designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA.\u003cspan\u003e \u003c\/span\u003eRecom ProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003econtains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. Our\u003cspan\u003e \u003c\/span\u003eFOCUSâ„¢ ProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003eis designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"G Biosciences","offers":[{"title":"1ml","offer_id":18640907534403,"sku":"786-238","price":376.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/tcm_99b13774-3830-4710-9641-ca45c066df5c.png?v=1745802081"},{"product_id":"786-239","title":"TCM Protease Arrestâ„¢","description":"\u003cp align=\"left\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/files\/AS_Protease_inhibitors_GBioscience_v1.pdf?v=1631168776\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/files\/AS_Protease_inhibitors_GBioscience_v1.pdf?v=1631168776\"\u003e\u003cstrong\u003e**Click Here to Download Protease Inhibitor Cocktail Selection Guide**\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp align=\"left\"\u003eA broad range, 200X concentrated, sterile filtered, ready-to-use protease inhibitor cocktail for the protection of proteins secreted into tissue culture media. TCM ProteaseArrestâ„¢ features optimized concentrations of Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A that inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases  trypsin-like and acid proteases. The inhibitor cocktail is designed to protect secreted proteins during cell culture for up to 48 hours.\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"0\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Type \u0026amp; key features\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInhibition Specificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e1X Composition\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration \u0026amp; Available Sizes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eFor protection of secreted proteins in tissue culture media.\u003c\/p\u003e\n\u003cp\u003eSterile filtered\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eA wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e0.6µM Aprotinin\u003c\/p\u003e\n\u003cp\u003e58µM Bestatin\u003c\/p\u003e\n\u003cp\u003e28µM E-64\u003c\/p\u003e\n\u003cp\u003e10.5µM Leupeptin\u003c\/p\u003e\n\u003cp\u003e2.1µM Pepstatin A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e200X Sterile filtered DMSO solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNo proprietary ingredients, fully disclosed formulation of five broad range protease inhibitors, including Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A\u003c\/li\u003e\n\u003cli\u003eBroad range protease inhibitor cocktail\u003c\/li\u003e\n\u003cli\u003eStable for up to 48 hours in cell culture media\u003c\/li\u003e\n\u003cli\u003eSterile filtered\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProtect secreted proteins from proteolytic degradation during cell culture \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eProteaseArrest Family\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003eOur\u003cspan\u003e \u003c\/span\u003eProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003eis a highly efficient, liquid format general protease inhibitor cocktail that offers \u0026gt;95% protease inhibition. The ProteaseArrestâ„¢ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species.  In addition to Bacteria, species-specific cocktails are available for\u003cspan\u003e \u003c\/span\u003eBacterial, Plant, Yeast,\u003cspan\u003e \u003c\/span\u003eFungus and\u003cspan\u003e \u003c\/span\u003eMammalian\u003cspan\u003e \u003c\/span\u003esamples. Our\u003cspan\u003e \u003c\/span\u003eRecom ProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003eis designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA.\u003cspan\u003e \u003c\/span\u003eRecom ProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003econtains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. Our\u003cspan\u003e \u003c\/span\u003eFOCUSâ„¢ ProteaseArrestâ„¢\u003cspan\u003e \u003c\/span\u003eis designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"G Biosciences","offers":[{"title":"2ml","offer_id":18640907599939,"sku":"786-239","price":730.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/tcm.png?v=1745802080"},{"product_id":"786-24","title":"Developing Trays","description":"","brand":"G Biosciences","offers":[{"title":"2","offer_id":18640907632707,"sku":"786-24","price":58.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/chemicals_14_d34337d2-08f9-45d3-87ac-78209bd33fac.png?v=1745802080"},{"product_id":"786-240","title":"FOCUSâ„¢ FASTsilverâ„¢","description":"","brand":"G Biosciences","offers":[{"title":"25 Mini Gels","offer_id":18640909107267,"sku":"786-240","price":571.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/chemicals_13_56cd6364-d722-43eb-8d21-c5b3a906ba61.png?v=1745802080"},{"product_id":"786-240t","title":"FOCUSâ„¢ FASTsilverâ„¢","description":"","brand":"G Biosciences","offers":[{"title":"5 Mini Gels","offer_id":18640909402179,"sku":"786-240T","price":161.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/chemicals_12_810e8a98-5c2f-4984-8bd2-49d741fc9584.png?v=1745802079"},{"product_id":"786-241","title":"InGel Silver","description":"","brand":"G Biosciences","offers":[{"title":"For 100 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