{"title":"DRY ICE FREIGHT","description":"","products":[{"product_id":"c300c105","title":"TransforMax-EPI300-Chemically Competent E. coli, 10 X 50 µl","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eTransforMax™ EPI300™ Electrocompetent and Chemically Competent E. coli\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eStandard cloning for large inserts and Gibson assembly electroporation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eClone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.\u003c\/li\u003e\n\u003cli\u003eGenerate clones with inducible copy number using CopyControl™ vectors.\u003c\/li\u003e\n\u003cli\u003eAchieve high transformation efficiencies: \u0026gt;1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC19 DNA.\u003c\/li\u003e\n\u003cli\u003eIncrease Gibson Assembly® colony counts\u003cspan\u003e \u003c\/span\u003ewith high-efficiency electroporation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGeneration of inducible copy-number clones using the CopyControl™ Cloning System.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"5\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" valign=\"top\"\u003e\n\u003cp\u003eTransforMax™ EPI300™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecells lack the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etonA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene and are engineered for use with Epicentre's CopyControl™ cDNA, Gene, and PCR Cloning Kit and other CopyControl Cloning Systems* that do not require phage T1-resistant cells. The cells contain an inducible mutant\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etrfA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene whose gene product is required for initiation of replication from the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eori\u003c\/em\u003eV origin of replication, such as that in CopyControl pCC1™ vectors. On LB chloramphenicol plates or in LB or SOC media supplemented with chloramphenicol, CopyControl clones grown in TransforMax EPI300\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ereplicate at single-copy number from the F-factor replicon because expression of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etrfA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene is repressed. Addition of CopyControl Induction Solution induces the cells to express the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etrfA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene product and induces their replication at high-copy number from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eori\u003c\/em\u003eV (Fig. 1). Replication from\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eori\u003c\/em\u003eV results in higher yields and higher purity of cloned DNA.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eBenefits\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCopy-number of clones is under tight control of an inducible promoter linked to the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etrfA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene.\u003c\/li\u003e\n\u003cli\u003eHigh transformation efficiency with clones of all sizes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003elacZΔM15\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003efor blue\/white screening of recombinants.\u003c\/li\u003e\n\u003cli\u003eRestriction-minus [\u003cem\u003emcrA, Δ(mrr-hsdRMS-mcrBC\u003c\/em\u003e)] phenotype enables efficient cloning of methylated DNA.\u003c\/li\u003e\n\u003cli\u003eEndonuclease-minus (\u003cem\u003eendA1\u003c\/em\u003e), to ensure high yields of DNA.\u003c\/li\u003e\n\u003cli\u003eRecombination-minus (\u003cem\u003erecA1\u003c\/em\u003e), for greater stability of large insert clones.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"5\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cimg alt=\"Copy number of CopyControl™ BAC clones can be induced 10- to 20-fold in TransforMax™ EPI300™ Chemically Competent E. coli\" title=\"i-TransforMax_BACClones\" src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/i-transformax_bacclones.gif?sfvrsn=0.7810071297198808\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cstrong\u003eFigure 1. Copy number of CopyControl™ BAC clones can be induced 10- to 20-fold in TransforMax™ EPI300™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli.\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eThe yield of BAC DNA from CopyControl BAC clones of 110-145 kb increased \u0026gt;14-fold following addition of CopyControl Induction Solution. U=uninduced cells; I=induced cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eGenotype\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eF\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003emcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003erpsL (Str\u003csup\u003eR\u003c\/sup\u003e) nupG trfA dhfr\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransforMax EPI300 Electrocompetent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;1 x 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTransforMax EPI300 Chemically Competent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;5 x 10\u003csup\u003e8\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003e*Covered by issued and\/or pending patents\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"10 X 50 µl","offer_id":18490487636035,"sku":"C300C105","price":978.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2e_a23fd303-25f9-4463-96ab-c4633deaa201.jpg?v=1736396391"},{"product_id":"c400ch10","title":"CopyCutter-EPI400-Chemically Competent E. coli, 10 rxns","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eCopyCutter™ EPI400™ Electrocompetent and Chemically Competent E. coli\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eOptimized for cloning toxic or unstable DNA\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStabilize toxic inserts in common cloning and expression vectors (pUC and pET-type vectors)\u003c\/li\u003e\n\u003cli\u003eClone and maintain challenging sequences at reduced plasmid copy number, then induce to high copy number for DNA recovery\u003c\/li\u003e\n\u003cli\u003eAvoid T1 and T5 phage contamination with tonA mutation\u003c\/li\u003e\n\u003cli\u003eChoose chemically competent cells for general cloning or electrocompetent cells for demanding applications such as library generation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCloning of unstable DNA sequences or those expressing toxic proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eCopyCutter™ EPI400™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure (Fig. 2).\u003csup\u003e1\u003c\/sup\u003e\u003c\/p\u003e\n\u003cp\u003eThe CopyCutter EPI400 cell line was derived from Epicentre's high-transformation efficiency phage T1-resistant TransforMAX™ EC100™-T1\u003csup\u003eR\u003c\/sup\u003e\u003cem\u003eE.coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors). This constitutively expressed gene,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epcnB\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e(plasmid copy number), was deleted from the TransforMAX EC100 strain and replaced with a modified\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epcnB\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene that is linked to an inducible promoter, creating the CopyCutter EPI400 strain.\u003c\/p\u003e\n\u003cp\u003eThe copy number of ColE1-type vectors in the CopyCutter EPI400 strain compared to the parental TransforMAX EC100 strain is approximately 4- to 25-fold lower, depending on the vector. Moreover, a short incubation in the presence of the CopyCutter Induction Solution can increase the copy number of the vector to improve plasmid yields (Fig. 3).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Copy-number of ColE1-type plasmids is lowered up to 25-fold in CopyCutter™ EPI400™ E. coli cells\" src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/g_copycutter_fig1.jpg?sfvrsn=0.1852300850148404\" title=\"g_CopyCutter_fig1\"\u003e\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1. Copy-number of ColE1-type plasmids is lowered up to 25-fold in CopyCutter™ EPI400™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecells.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLanes C, TransforMax™ EC100™ cells; Lanes U and I, uninduced or induced CopyCutter EPI400 cells. DNA extracts from the same number of lysed cells (based on OD\u003csub\u003e600\u003c\/sub\u003e) were loaded per lane.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/i_copycutter_dnainserts.gif?sfvrsn=0.3391340217580109\" alt=\"DNA inserts encoding toxic gene products successfully cloned into high-copy-number vectors using CopyCutter™ Competent E. coli cells\"\u003e\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2. DNA inserts encoding toxic gene products were successfully cloned into high-copy-number vectors using CopyCutter™ EPI400™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecells.\u003c\/strong\u003e\u003cspan\u003e After sequencing, the full-length \u003c\/span\u003e\u003cem\u003eacpP\u003c\/em\u003e\u003cspan\u003e clones in TransforMAX™ EC100™ cells were found to contain multiple point mutations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/g_copycutter_uninduced.jpg?sfvrsn=0.435955505080011\" alt=\"Uninduced CopyCutter™ EPI400™ E. coli cells containing a regB clone (lane U) are induced to higher-copy number (lane I) using the CopyCutter Induction Solution\"\u003e\u003cstrong\u003eFigure 3. Uninduced CopyCutter™ EPI400™ \u003cem\u003eE. coli\u003c\/em\u003e cells containing a \u003cem\u003eregB\u003c\/em\u003e clone (lane U) are induced to higher-copy number (lane I) using the CopyCutter Induction Solution.\u003c\/strong\u003e Crude extracts of plasmid DNA were prepared from cells grown in selective media and analyzed by agarose gel electrophoresis. Approximately the same number of lysed cells (based on A\u003csub\u003e600\u003c\/sub\u003e) were loaded per lane.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBenefits\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMaintain clones at low-copy number, then induce to higher copy number for improved plasmid yield.\u003c\/li\u003e\n\u003cli\u003eHigh transformation efficiency with clones of all sizes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003etonA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003efor resistance to bacteriophages T1 and T5.\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003elacZΔM15\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003efor blue\/white screening of recombinants.\u003c\/li\u003e\n\u003cli\u003eRestriction minus [\u003cem\u003emcrA, Δ(mrr-hsdRMS-mcrBC)\u003c\/em\u003e] genotype enables efficient cloning of methylated DNA.\u003c\/li\u003e\n\u003cli\u003eEndonuclease minus (\u003cem\u003eendA1\u003c\/em\u003e) to ensure high yields of DNA.\u003c\/li\u003e\n\u003cli\u003eRecombination minus (\u003cem\u003erecA1\u003c\/em\u003e) for greater stability of large cloned inserts.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eGenotype\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eF\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003emcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003erpsL (Str\u003csup\u003eR\u003c\/sup\u003e) nupG trfA tonA pcnB4 dhfr\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCopyCutter EPI400 Electrocompetent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;1 x 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCopyCutter EPI400 Chemically Competent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;1 x 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eHaskins, D. (2004)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eEpicentre Forum\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003cstrong\u003e11(\u003c\/strong\u003e5), 6.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cem\u003e*Covered by issued and\/or pending patents.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"10 x 50 µl","offer_id":18490487668803,"sku":"C400CH10","price":1138.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_b883470d-54b2-4f13-93ea-1e21a934b781.jpg?v=1736396390"},{"product_id":"c400el10","title":"CopyCutter-EPI400-Electrocompetent E. coli, 10 x 50 µl","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eCopyCutter™ EPI400™ Electrocompetent and Chemically Competent E. coli\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eOptimized for cloning toxic or unstable DNA\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStabilize toxic inserts in common cloning and expression vectors (pUC and pET-type vectors)\u003c\/li\u003e\n\u003cli\u003eClone and maintain challenging sequences at reduced plasmid copy number, then induce to high copy number for DNA recovery\u003c\/li\u003e\n\u003cli\u003eAvoid T1 and T5 phage contamination with tonA mutation\u003c\/li\u003e\n\u003cli\u003eChoose chemically competent cells for general cloning or electrocompetent cells for demanding applications such as library generation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCloning of unstable DNA sequences or those expressing toxic proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eCopyCutter™ EPI400™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure (Fig. 2).\u003csup\u003e1\u003c\/sup\u003e\u003c\/p\u003e\n\u003cp\u003eThe CopyCutter EPI400 cell line was derived from Epicentre's high-transformation efficiency phage T1-resistant TransforMAX™ EC100™-T1\u003csup\u003eR\u003c\/sup\u003e\u003cem\u003eE.coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors). This constitutively expressed gene,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epcnB\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e(plasmid copy number), was deleted from the TransforMAX EC100 strain and replaced with a modified\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epcnB\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003egene that is linked to an inducible promoter, creating the CopyCutter EPI400 strain.\u003c\/p\u003e\n\u003cp\u003eThe copy number of ColE1-type vectors in the CopyCutter EPI400 strain compared to the parental TransforMAX EC100 strain is approximately 4- to 25-fold lower, depending on the vector. Moreover, a short incubation in the presence of the CopyCutter Induction Solution can increase the copy number of the vector to improve plasmid yields (Fig. 3).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Copy-number of ColE1-type plasmids is lowered up to 25-fold in CopyCutter™ EPI400™ E. coli cells\" src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/g_copycutter_fig1.jpg?sfvrsn=0.1852300850148404\" title=\"g_CopyCutter_fig1\"\u003e\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1. Copy-number of ColE1-type plasmids is lowered up to 25-fold in CopyCutter™ EPI400™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecells.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLanes C, TransforMax™ EC100™ cells; Lanes U and I, uninduced or induced CopyCutter EPI400 cells. DNA extracts from the same number of lysed cells (based on OD\u003csub\u003e600\u003c\/sub\u003e) were loaded per lane.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/i_copycutter_dnainserts.gif?sfvrsn=0.3391340217580109\" alt=\"DNA inserts encoding toxic gene products successfully cloned into high-copy-number vectors using CopyCutter™ Competent E. coli cells\"\u003e\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2. DNA inserts encoding toxic gene products were successfully cloned into high-copy-number vectors using CopyCutter™ EPI400™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecells.\u003c\/strong\u003e\u003cspan\u003e After sequencing, the full-length \u003c\/span\u003e\u003cem\u003eacpP\u003c\/em\u003e\u003cspan\u003e clones in TransforMAX™ EC100™ cells were found to contain multiple point mutations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Epicentre\/g_copycutter_uninduced.jpg?sfvrsn=0.435955505080011\" alt=\"Uninduced CopyCutter™ EPI400™ E. coli cells containing a regB clone (lane U) are induced to higher-copy number (lane I) using the CopyCutter Induction Solution\"\u003e\u003cstrong\u003eFigure 3. Uninduced CopyCutter™ EPI400™ \u003cem\u003eE. coli\u003c\/em\u003e cells containing a \u003cem\u003eregB\u003c\/em\u003e clone (lane U) are induced to higher-copy number (lane I) using the CopyCutter Induction Solution.\u003c\/strong\u003e Crude extracts of plasmid DNA were prepared from cells grown in selective media and analyzed by agarose gel electrophoresis. Approximately the same number of lysed cells (based on A\u003csub\u003e600\u003c\/sub\u003e) were loaded per lane.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBenefits\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMaintain clones at low-copy number, then induce to higher copy number for improved plasmid yield.\u003c\/li\u003e\n\u003cli\u003eHigh transformation efficiency with clones of all sizes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003etonA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003efor resistance to bacteriophages T1 and T5.\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003elacZΔM15\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003efor blue\/white screening of recombinants.\u003c\/li\u003e\n\u003cli\u003eRestriction minus [\u003cem\u003emcrA, Δ(mrr-hsdRMS-mcrBC)\u003c\/em\u003e] genotype enables efficient cloning of methylated DNA.\u003c\/li\u003e\n\u003cli\u003eEndonuclease minus (\u003cem\u003eendA1\u003c\/em\u003e) to ensure high yields of DNA.\u003c\/li\u003e\n\u003cli\u003eRecombination minus (\u003cem\u003erecA1\u003c\/em\u003e) for greater stability of large cloned inserts.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eGenotype\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eF\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003emcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003erpsL (Str\u003csup\u003eR\u003c\/sup\u003e) nupG trfA tonA pcnB4 dhfr\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCopyCutter EPI400 Electrocompetent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;1 x 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCopyCutter EPI400 Chemically Competent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;1 x 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eHaskins, D. (2004)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eEpicentre Forum\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003cstrong\u003e11(\u003c\/strong\u003e5), 6.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cem\u003e*Covered by issued and\/or pending patents.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"10 x 50 µl","offer_id":18490487701571,"sku":"C400EL10","price":1333.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2b_5a491230-df45-40f5-8f5c-6861fc670982.jpg?v=1736396389"},{"product_id":"cc02810","title":"TransforMax EC100 Chemically Competent E. coli, 10 x 50 µl","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eTransforMax™ EC100™ Electrocompetent  and Chemically Competent E. coli\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eGeneral cloning strain\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eClone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.\u003c\/li\u003e\n\u003cli\u003eAchieve high efficiencies with convenient chemically competent format: \u0026gt;5 × 10\u003csup\u003e8\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC19 DNA.\u003c\/li\u003e\n\u003cli\u003eIdeal for cloning, subcloning and plasmid preparation due to endA and recA mutations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine cloning of DNA up to 200 kb.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe highly versatile TransforMax™ EC100™\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBenefits\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh transformation efficiency with clones of all sizes, including BAC clones (Table 1).\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003elacZΔM15\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003efor blue\/white screening of recombinants.\u003c\/li\u003e\n\u003cli\u003eRestriction minus [\u003cem\u003emcrA, Δ(mrr-hsdRMS-mcrBC)\u003c\/em\u003e] enables efficient cloning of methylated DNA.\u003c\/li\u003e\n\u003cli\u003eEndonuclease minus (\u003cem\u003eendA1\u003c\/em\u003e) to ensure high yields of DNA.\u003c\/li\u003e\n\u003cli\u003eRecombination minus (\u003cem\u003erecA1\u003c\/em\u003e) for greater stability of large cloned inserts.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"5\" border=\"1\" align=\"center\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"table_header\" align=\"center\"\u003e\u003cstrong\u003eDNA\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"table_header\" align=\"center\"\u003e\u003cstrong\u003eTransforMax™ EC100™ Chemically Competent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"table_header\" align=\"center\"\u003e\u003cstrong\u003eTransforMax™ EC100™ Electrocompetent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003epUC19\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e1.4 × 10\u003csup\u003e8\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e1.4 × 10\u003csup\u003e10\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003e8.1-kb Clone\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e1.3 × 10\u003csup\u003e7\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003eNot tested\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003e13.1-kb Clone\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e4.3 × 10\u003csup\u003e6\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e1.3 × 10\u003csup\u003e9\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003e23.1-kb Clone\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e9.2 × 10\u003csup\u003e5\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e3.0 × 10\u003csup\u003e8\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003e145-kb BAC Clone\u003c\/td\u003e\n\u003ctd align=\"center\"\u003eNot tested\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e7 × 10\u003csup\u003e7\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"center\"\u003e13.1-kb clone directly from a ligation reaction\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e2.2 × 10\u003csup\u003e5\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e2.1 × 10\u003csup\u003e7\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1. Comparison of the transformation efficiencies of TransforMax™ EC100™\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ewith a variety of DNAs.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eTransformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu\/µg of DNA and are the average transformation efficiencies obtained from several trials.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGenotype\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eF\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003emcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ\u003csup\u003e-\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003erpsL (Str\u003csup\u003eR\u003c\/sup\u003e) nupG\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTransforMax EC100 Electrocompetent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;1 × 10\u003csup\u003e10\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTransforMax EC100 Chemically Competent\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransformation efficiency of \u0026gt;5 × 10\u003csup\u003e8\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003ecfu\/µg of pUC19.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"10 x 50 µl","offer_id":18490487734339,"sku":"CC02810","price":822.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2a_89b39dea-79db-431a-92fb-aacf4ad66c05.jpg?v=1736396389"},{"product_id":"ccis125","title":"CopyControl-Induction Solution, 25 mL","description":"\u003cp\u003eCopyControl-Induction Solution, 25 mL\u003c\/p\u003e","brand":"Lucigen","offers":[{"title":"25ml","offer_id":18490487832643,"sku":"CCIS125","price":366.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2e_61300140-087c-474e-8c4d-6a0ab62227ad.jpg?v=1736396388"},{"product_id":"14000-1","title":"NxSeq-AmpFREE Low DNA Library Kit, 12 Reactions","description":"\u003cp\u003e\u003cstrong\u003eThe highest efficiency, low input, pcr-free fragment library prep kit available at the lowest cost.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow Input\u003c\/strong\u003e: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Efficiency: \u003c\/strong\u003eOptimized adaptor ligation\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eproduces more sequenceable fragments in each library, yielding better coverage \u0026amp; depth from single or multiplexed libraries.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePCR-free\u003c\/strong\u003e\u003cstrong\u003e:\u003c\/strong\u003e Prevents the introduction of PCR-bias, providing more uniform coverage.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFast: \u003c\/strong\u003e2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAffordable:\u003c\/strong\u003e Best priced and best performing kit available\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThe NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eEach\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eNxSeq AmpFREE Low DNA Library Kit\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003econtains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eEach box of NxSeq Adaptors\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003econtains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.\u003c\/p\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490495041603,"sku":"14000-1","price":1132.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_14_216e2b5a-13a0-452a-b692-f89faf2f624d.png?v=1736396335"},{"product_id":"14000-2","title":"NxSeq-AmpFREE Low DNA Library Kit, 48 Reactions","description":"\u003cp\u003e\u003cstrong\u003eThe highest efficiency, low input, pcr-free fragment library prep kit available at the lowest cost.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow Input\u003c\/strong\u003e: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Efficiency: \u003c\/strong\u003eOptimized adaptor ligation\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eproduces more sequenceable fragments in each library, yielding better coverage \u0026amp; depth from single or multiplexed libraries.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePCR-free\u003c\/strong\u003e\u003cstrong\u003e:\u003c\/strong\u003e Prevents the introduction of PCR-bias, providing more uniform coverage.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFast: \u003c\/strong\u003e2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAffordable:\u003c\/strong\u003e Best priced and best performing kit available\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThe NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eEach\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eNxSeq AmpFREE Low DNA Library Kit\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003econtains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eEach box of NxSeq Adaptors\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003econtains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.\u003c\/p\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490495074371,"sku":"14000-2","price":3914.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_15_3a6f7b8d-bc69-41c4-a512-40eb9fd9b9d1.png?v=1736396335"},{"product_id":"14300-1","title":"NxSeq-Adaptors, Box 1","description":"","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490495107139,"sku":"14300-1","price":859.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_16_a885161e-23ba-4230-a79b-557eaf04de0c.png?v=1736396334"},{"product_id":"14400-1","title":"NxSeq-Adaptors, Box 2","description":"","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490495139907,"sku":"14400-1","price":859.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_17_d6215107-9bf2-491e-a256-dbf8215b7e5b.png?v=1736396334"},{"product_id":"15012-1","title":"NxSeq-UltraLow DNA Library Kit, 12 Reactions","description":"\u003cp\u003e\u003cstrong\u003eLowest input, highest efficiency, Illumina-compatible DNA fragment library prep kit\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Quality Data\u003c\/strong\u003e: High efficiency adapter ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSensitive:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eConstruct DNA fragment libraries from as little as 50 pg to as much 75 ng of sheared\/fragmented DNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMinimal Bias\u003c\/strong\u003e: Robust, uniform PCR amplification improves coverage uniformity when working with low input amounts of genomic DNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible:\u003c\/strong\u003eExtensively tested in de novo whole genome sequencing or resequencing, but compatible with other applications such as exome-seq, ChIP-seq and FFPE DNA samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFast\u003c\/strong\u003e: 3 hour protocol gets your samples on the sequencer quicker\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Value\u003c\/strong\u003e: Cost-effective library and indexing kits which produce excellent sequencing results\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e The NxSeq® UltraLow DNA Library Kit and NxSeq Single Indexing Kits are only compatible with Illumina sequencers.\u003c\/p\u003e\n\u003cbr\u003eThe NxSeq UltraLow DNA Library Kit, 12 Reactions contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG), 2X PCR Master Mix (MM) and Elution Buffer (EB). At least one NxSeq Single Indexing Kit is required to complete library prep and must be purchased separately.\u003cbr\u003e\u003cbr\u003eEach NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. Each NxSeq® Single Index equals the TruSeq® LT Index with the same number.","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490495172675,"sku":"15012-1","price":1181.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_18_cb7c8014-1afc-4e84-b5d4-462bfec11199.png?v=1736396333"},{"product_id":"15096-1","title":"NxSeq-UltraLow DNA Library Kit, 96 Reactions","description":"\u003cp\u003e\u003cstrong\u003eHigh-throughput optimized, dual-indexed DNA fragment library prep for Illumina sequencers\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFormatted for High Throughput:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eProvides sufficient overfill of each reagent to enable automated or manual multi-channel pipet-based 96-well plate library construction\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEasy-to-use:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eTwo color dual indexing primer sets facilitate visual verification of correct primer dispensing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSame performance features as the\u003cspan\u003e \u003c\/span\u003eNxSeq® UltraLow DNA Library Kit, 12 Reactions\u003cspan\u003e \u003c\/span\u003eincluding:\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eProduces complex libraries with improved sequencing depth uniformity and coverage\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRequires only 50 pg to 75 ng input of sheared\/fragmented DNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMinimizes PCR bias to improve coverage uniformity\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWell suited for de novo whole genome sequencing or re-sequencing and compatible with other applications such as exome-seq, ChIP-seq and FFPE and cell free DNA samples\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Value:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eCost-effective library and indexing kits that produce excellent sequencing results\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe NxSeq® UltraLow DNA Library Kit, 96 Reactions and NxSeq® HT Dual Indexing Kit are only compatible with Illumina sequencers.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eThe NxSeq® UltraLow DNA Library Kit, 96 Reactions contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG), 2X PCR Master Mix (MM) and Elution Buffer (EB). A NxSeq® HT Dual Indexing Kit is required to complete library prep and must be purchased separately.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eThe NxSeq® HT Dual Indexing Kit contains a Universal Adaptor, Adaptor Dilution Buffer and Indexing Primers 701 – 712, and 501 – 508. There is enough of each reagent in this kit to generate 96 different dual indexed libraries. 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The enzyme is capable of up to 70,000 insertions per binding event.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"category-description\"\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eA recombinant\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.\u003c\/p\u003e\n\u003ctable width=\"400\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Concentration\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"tables-figures-07-5-charts-body-8-9\"\u003e10,000 U\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurity (SDS-PAGE)\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"tables-figures-07-5-charts-body-8-9\"\u003e\u0026gt;99%\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSS Exonuclease\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003eFunctional\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e16S rDNA Contamination\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003e100 U \u0026lt;10 copies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndonuclease\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003e100 U \u0026lt;10% converted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003e-20\u0026amp;°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"subcat-tabs\"\u003e\n\u003cdiv class=\"resp-tabs-container\"\u003e\n\u003cdiv class=\"resp-tab-content resp-tab-content-active\" aria-labelledby=\"tab_item-0\"\u003e\n\u003cdiv class=\"product-tab-ordering\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490496876611,"sku":"30221-1","price":756.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_17_df8cdff4-2d6b-4991-99ef-d59db52961ce.png?v=1736396318"},{"product_id":"30221-2","title":"NxGen-phi29 DNA Polymerase, 10,000 U","description":"\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eExcellent strand displacement, fidelity and speed.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/NxGen-Enzymes\/icon-NxG-small.png\" alt=\"NxGen Enzymes Icon\" width=\"34\" height=\"34\" class=\"luc-float-left\"\u003ephi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"category-description\"\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eA recombinant\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.\u003c\/p\u003e\n\u003ctable width=\"400\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Concentration\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"tables-figures-07-5-charts-body-8-9\"\u003e10,000 U\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurity (SDS-PAGE)\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"tables-figures-07-5-charts-body-8-9\"\u003e\u0026gt;99%\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSS Exonuclease\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003eFunctional\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e16S rDNA Contamination\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003e100 U \u0026lt;10 copies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndonuclease\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003e100 U \u0026lt;10% converted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd class=\"info-table-cells\"\u003e-20\u0026amp;°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490496909379,"sku":"30221-2","price":2558.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_18_844a1785-c998-4c14-8ce0-ce46e2660842.png?v=1736396318"},{"product_id":"30222-1","title":"NxGen-M-MuLV Reverse Transcriptase, 50,000 U","description":"\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eOutstanding cDNA synthesis.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/NxGen-Enzymes\/icon-NxG-small.png\" width=\"34\" height=\"34\" alt=\"NxGen Enzymes Icon\"\u003e\u003cspan\u003e \u003c\/span\u003eM-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)\/oligo (dT) as a substrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eA recombinant\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.\u003c\/p\u003e\n\u003ctable width=\"400\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Concentration\u003c\/td\u003e\n\u003ctd\u003e200,000 U\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurity (SDS-PAGE)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSS Exonuclease\u003c\/td\u003e\n\u003ctd\u003e2,000 U \u0026lt;5.0% released\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDS Exonuclease\u003c\/td\u003e\n\u003ctd\u003e2,000 U \u0026lt;0.5% released\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndonuclease\u003c\/td\u003e\n\u003ctd\u003e2,000 U \u0026lt;10% converted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e16S rDNA Contamination\u003c\/td\u003e\n\u003ctd\u003e2,000 U \u0026lt;10 copies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490496942147,"sku":"30222-1","price":731.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_1_5edf7b75-5fd3-4145-a540-9687af4d5fd5.png?v=1736396317"},{"product_id":"30223-1","title":"NxGen-T7 RNA Polymerase, 25,000 U","description":"\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eReliable transcription\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/NxGen-Enzymes\/icon-NxG-small.png\" width=\"34\" height=\"34\" alt=\"NxGen Enzymes Icon\"\u003eT7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eOne unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ePurified from a strain of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003ethat expresses the recombinant T7 RNA Polymerase gene.\u003c\/p\u003e\n\u003ctable width=\"400\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Concentration\u003c\/td\u003e\n\u003ctd\u003e50,000 U\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurity (SDS-PAGE)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSS Exonuclease\u003c\/td\u003e\n\u003ctd\u003e500 U \u0026lt;0.1% released\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDS Exonuclease\u003c\/td\u003e\n\u003ctd\u003e500 U \u0026lt;0.1% released\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndonuclease\u003c\/td\u003e\n\u003ctd\u003e500 U \u0026lt;10% converted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNAse Contamination\u003c\/td\u003e\n\u003ctd\u003e500 U = none detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e16S rDNA Contamination\u003c\/td\u003e\n\u003ctd\u003e500 U \u0026lt;10 copies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490497007683,"sku":"30223-1","price":682.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_3_6f0cdde0-4247-4e38-9e5a-b5f1a02fb90b.png?v=1736396316"},{"product_id":"30241-1","title":"NxGen-T4 DNA Ligase (Low Concentration), 1,500 U","description":"\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eReliably join double-stranded nucleic acid chains.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/NxGen-Enzymes\/icon-NxG-small.png\" alt=\"NxGen Enzymes Icon\" width=\"34\" height=\"34\" class=\"luc-float-left\"\u003eT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA\/RNA hybrids.\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" class=\"table table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eT4 DNA Ligase Version\u003c\/td\u003e\n\u003ctd\u003eSizes\u003c\/td\u003e\n\u003ctd\u003eLigase Concentration\u003c\/td\u003e\n\u003ctd\u003eProvided Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLow Concentration Ligase\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1,500 U\u003cbr\u003e7,500 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e2 U\/µL\u003c\/td\u003e\n\u003ctd\u003e10X T4 DNA Ligase Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHigh Concentration Rapid Kit\u003c\/td\u003e\n\u003ctd\u003e1,500 U\u003cbr\u003e7,500 U\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e10 U\/µL\u003c\/td\u003e\n\u003ctd\u003e2X Rapid Ligation Buffer,\u003cbr\u003e10X T4 DNA Ligase Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.\u003c\/p\u003e\n\u003ctable width=\"128\" border=\"0\" cellpadding=\"2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/T4-DNA-Ligase\/Fig-1-T4-DNA-Ligase-Purity.png\" width=\"100\" height=\"255\" alt=\"NxGen T4 DNA Ligase Purity\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e \u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1:  Purity\u003c\/strong\u003e\u003cbr\u003eThe purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining.  Molecular weight markers are in Lane 1.  T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.\u003c\/p\u003e\n\u003ctable width=\"546\" border=\"0\" cellpadding=\"2\" height=\"438\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 534.943px;\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/T4-DNA-Ligase\/Fig-2-T4-DNA-Ligase-Activity-Exonuclease-Endonuclease.png\" width=\"172\" height=\"237\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 534.943px;\"\u003e\n\u003cp class=\"figure\"\u003e \u003cstrong\u003eFigure 2: \u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eExonuclease and Endonuclease Activity Assay\u003c\/p\u003e\n\u003cp class=\"figure\"\u003eThe T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6).  Molecular weight markers are in Lanes 1 and 2.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eOne Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of\u003cspan\u003e \u003c\/span\u003e\u003csup\u003e32\u003c\/sup\u003eP-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eA recombinant\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain carrying the cloned T4 DNA Ligase gene.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490497073219,"sku":"30241-1","price":630.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_5_ac287fc6-8d77-466f-a995-9bed1d4c28b4.png?v=1736396315"},{"product_id":"30243-1","title":"NxGen-T4 DNA Ligase (High Concentration), 1,500 U","description":"\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eReliably join double-stranded nucleic acid chains.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/NxGen-Enzymes\/icon-NxG-small.png\" alt=\"NxGen Enzymes Icon\" width=\"34\" height=\"34\" class=\"luc-float-left\"\u003eT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA\/RNA hybrids.\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" class=\"table table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eT4 DNA Ligase Version\u003c\/td\u003e\n\u003ctd\u003eSizes\u003c\/td\u003e\n\u003ctd\u003eLigase Concentration\u003c\/td\u003e\n\u003ctd\u003eProvided Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLow Concentration Ligase\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1,500 U\u003cbr\u003e7,500 U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e2 U\/µL\u003c\/td\u003e\n\u003ctd\u003e10X T4 DNA Ligase Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHigh Concentration Rapid Kit\u003c\/td\u003e\n\u003ctd\u003e1,500 U\u003cbr\u003e7,500 U\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e10 U\/µL\u003c\/td\u003e\n\u003ctd\u003e2X Rapid Ligation Buffer,\u003cbr\u003e10X T4 DNA Ligase Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.\u003c\/p\u003e\n\u003ctable width=\"128\" border=\"0\" cellpadding=\"2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/T4-DNA-Ligase\/Fig-1-T4-DNA-Ligase-Purity.png\" width=\"100\" height=\"255\" alt=\"NxGen T4 DNA Ligase Purity\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e \u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1:  Purity\u003c\/strong\u003e\u003cbr\u003eThe purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining.  Molecular weight markers are in Lane 1.  T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.\u003c\/p\u003e\n\u003ctable width=\"546\" border=\"0\" cellpadding=\"2\" height=\"438\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/T4-DNA-Ligase\/Fig-2-T4-DNA-Ligase-Activity-Exonuclease-Endonuclease.png\" width=\"172\" height=\"237\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e \u003cstrong\u003eFigure 2: \u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eExonuclease and Endonuclease Activity Assay\u003c\/p\u003e\n\u003cp class=\"figure\"\u003eThe T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6).  Molecular weight markers are in Lanes 1 and 2.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnit Definition:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eOne Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of\u003cspan\u003e \u003c\/span\u003e\u003csup\u003e32\u003c\/sup\u003eP-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eA recombinant\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003estrain carrying the cloned T4 DNA Ligase gene.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490497138755,"sku":"30243-1","price":893.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_7_044e8523-4e12-4bcd-a41b-6fe4ddb99b15.png?v=1736396314"},{"product_id":"30281-1","title":"NxGen-RNAse Inhibitor, 10,000 U","description":"","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490497204291,"sku":"30281-1","price":957.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_9_9de82a4f-4cb1-41ff-8888-88b18d239671.png?v=1736396313"},{"product_id":"30281-2","title":"NxGen-RNAse Inhibitor, 50,000 U","description":"","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490497237059,"sku":"30281-2","price":2132.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/mid_level_10_99002117-67e8-497b-8e69-c67b46de10c1.png?v=1736396313"},{"product_id":"60051-1","title":"E. cloni-10G ELITE Electrocompetent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G and 10GF' Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies (5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eelectrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003ch2\u003eChoice of strain:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eLibrary construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10GF′Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eContain the F' plasmid for infection with M13 to produce ssDNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eChoice of efficiency:\u003c\/h2\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cspan\u003e \u003c\/span\u003e2\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e5\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid cloning.\u003c\/strong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"562\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\" width=\"265\"\u003e\n\u003ch2\u003e\u003cimg alt=\"DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells\" border=\"0\" height=\"158\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Recombinant-Yields.gif\" width=\"227\"\u003e\u003c\/h2\u003e\n\u003c\/td\u003e\n\u003ctd width=\"25\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"265\"\u003e\n\u003cp\u003e\u003cimg alt=\"Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)\" border=\"0\" height=\"150\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Transformation-Efficiency.gif\" width=\"241\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003eTransformation efficiency comparison of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cul\u003e\u003c\/ul\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490499727427,"sku":"60051-1","price":1143.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2e_edfbd06e-b73a-4ba2-8992-1737b0115eec.jpg?v=1736396296"},{"product_id":"60052-1","title":"E. cloni-10G ELITE Electrocompetent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G and 10GF' Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies (5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eelectrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003ch2\u003eChoice of strain:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eLibrary construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10GF′Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eContain the F' plasmid for infection with M13 to produce ssDNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eChoice of efficiency:\u003c\/h2\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cspan\u003e \u003c\/span\u003e2\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e5\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid cloning.\u003c\/strong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"562\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\" width=\"265\"\u003e\n\u003ch2\u003e\u003cimg alt=\"DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells\" border=\"0\" height=\"158\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Recombinant-Yields.gif\" width=\"227\"\u003e\u003c\/h2\u003e\n\u003c\/td\u003e\n\u003ctd width=\"25\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"265\"\u003e\n\u003cp\u003e\u003cimg alt=\"Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)\" border=\"0\" height=\"150\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Transformation-Efficiency.gif\" width=\"241\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003eTransformation efficiency comparison of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490499792963,"sku":"60052-1","price":1050.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953392468035,"sku":"60052-2","price":1526.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_45fe99c7-3750-412d-b39a-d912fbe15a54.jpg?v=1736396296"},{"product_id":"60052-3","title":"E. cloni-10G ELITE Electrocompetent Cells (SixPacks)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G and 10GF' Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies (5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eelectrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003ch2\u003eChoice of strain:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eLibrary construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10GF′Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eContain the F' plasmid for infection with M13 to produce ssDNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eChoice of efficiency:\u003c\/h2\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cspan\u003e \u003c\/span\u003e2\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e5\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid cloning.\u003c\/strong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"562\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\" width=\"265\"\u003e\n\u003ch2\u003e\u003cimg alt=\"DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells\" border=\"0\" height=\"158\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Recombinant-Yields.gif\" width=\"227\"\u003e\u003c\/h2\u003e\n\u003c\/td\u003e\n\u003ctd width=\"25\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"265\"\u003e\n\u003cp\u003e\u003cimg alt=\"Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)\" border=\"0\" height=\"150\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Transformation-Efficiency.gif\" width=\"241\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003eTransformation efficiency comparison of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"24 rxns","offer_id":18490499858499,"sku":"60052-3","price":1264.0,"currency_code":"AUD","in_stock":true},{"title":"48 rxns","offer_id":31953392861251,"sku":"60052-4","price":1842.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_66604a76-9789-4f0e-9316-2bc2818e82fd.jpg?v=1736396295"},{"product_id":"60061-1","title":"E. cloni-10GF' ELITE Electrocompetent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G and 10GF' Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies (5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eelectrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003ch2\u003eChoice of strain:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eLibrary construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10GF′Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eContain the F' plasmid for infection with M13 to produce ssDNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eChoice of efficiency:\u003c\/h2\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cspan\u003e \u003c\/span\u003e2\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e5\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid cloning.\u003c\/strong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"562\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\" width=\"265\"\u003e\n\u003ch2\u003e\u003cimg alt=\"DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells\" border=\"0\" height=\"158\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Recombinant-Yields.gif\" width=\"227\"\u003e\u003c\/h2\u003e\n\u003c\/td\u003e\n\u003ctd width=\"25\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"265\"\u003e\n\u003cp\u003e\u003cimg alt=\"Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)\" border=\"0\" height=\"150\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Transformation-Efficiency.gif\" width=\"241\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003eTransformation efficiency comparison of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490499956803,"sku":"60061-1","price":1056.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953313660995,"sku":"60061-2","price":1538.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2d_6a43120f-2eb0-470c-bd07-4e08eb2e3a42.jpg?v=1736396295"},{"product_id":"60080-1","title":"E. cloni-10G SUPREME Electrocompetent Cells (DUOs)","description":"\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500022339,"sku":"60080-1","price":1469.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953395580995,"sku":"60080-2","price":2282.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2d_214c6c32-e43e-43cc-acd0-146c28f00c77.jpg?v=1736396294"},{"product_id":"60081-1","title":"E. cloni-10G SUPREME Electrocompetent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G and 10GF' Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies (5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eelectrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003ch2\u003eChoice of strain:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eLibrary construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10GF′Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eContain the F' plasmid for infection with M13 to produce ssDNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eChoice of efficiency:\u003c\/h2\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cspan\u003e \u003c\/span\u003e2\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e5\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid cloning.\u003c\/strong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"562\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\" width=\"265\"\u003e\n\u003ch2\u003e\u003cimg alt=\"DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells\" border=\"0\" height=\"158\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Recombinant-Yields.gif\" width=\"227\"\u003e\u003c\/h2\u003e\n\u003c\/td\u003e\n\u003ctd width=\"25\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"265\"\u003e\n\u003cp\u003e\u003cimg alt=\"Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)\" border=\"0\" height=\"150\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Transformation-Efficiency.gif\" width=\"241\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003eTransformation efficiency comparison of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500087875,"sku":"60081-1","price":1563.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2a_56eeb2da-579a-4b38-975a-6f8807e0d495.jpg?v=1736396293"},{"product_id":"60106-1","title":"E. cloni-10G Chemically Competent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G Chemically Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of transformation efficiencies (1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 1 x 10\u003csup\u003e9\u003c\/sup\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency cells result in high colony counts\u003c\/li\u003e\n\u003cli\u003eEndorsed by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.lucigen.com\/docs\/SGI-App-Note-Featuring-Lucigen-10G-Comp-Cells-High-efficiency-Gibson-Assembly-Constructs.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eSGI-DNA for Gibson Assembly® application\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"category-description\"\u003e\n\u003cdiv\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.  \u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003echemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eRoutine cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/p\u003e\n\u003cp\u003eChoice of efficiency:\u003c\/p\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G Chemically Competent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA; (\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e8\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ein 96-well plates).\u003cbr\u003eHighly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G Chemically Competent Cells, Subcloning Grade\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA)\u003cstrong\u003e.  \u003c\/strong\u003e\u003cbr\u003eThe best value available anywhere for simple cloning and plasmid propagation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"195\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"128\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"101\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"216\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cp\u003e10G Chemically Competent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES: without IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cp\u003e10G Chemically Competent Subcloning Grade\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES: without IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eGenotypes\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eF-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003emcrA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eΔ(\u003cem\u003emrr-hsd\u003c\/em\u003eRMS-\u003cem\u003emcr\u003c\/em\u003eBC)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eend\u003c\/em\u003eA1\u003cspan\u003e \u003c\/span\u003e\u003cem\u003erec\u003c\/em\u003eA1 Φ80\u003cem\u003edlac\u003c\/em\u003eZΔM15 Δ\u003cem\u003elac\u003c\/em\u003eX74\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eara\u003c\/em\u003eD139 Δ(\u003cem\u003eara,leu\u003c\/em\u003e)7697\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egal\u003c\/em\u003eU\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egal\u003c\/em\u003eK\u003cspan\u003e \u003c\/span\u003e\u003cem\u003erps\u003c\/em\u003eL\u003cspan\u003e \u003c\/span\u003e\u003cem\u003enup\u003c\/em\u003eG λ-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eton\u003c\/em\u003eA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e 10G Chemically Competent Cells \u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 1 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA;\u003cbr\u003e\u003cstrong\u003e  96-well format\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 1 × 10\u003csup\u003e8\u003c\/sup\u003e in 96-well plates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500218947,"sku":"60106-1","price":950.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2d_317b82b5-1c48-4d4d-8af3-806ae00bf345.jpg?v=1736396293"},{"product_id":"60107-1","title":"E. cloni-10G Chemically Competent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G Chemically Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of transformation efficiencies (1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 1 x 10\u003csup\u003e9\u003c\/sup\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency cells result in high colony counts\u003c\/li\u003e\n\u003cli\u003eEndorsed by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.lucigen.com\/docs\/SGI-App-Note-Featuring-Lucigen-10G-Comp-Cells-High-efficiency-Gibson-Assembly-Constructs.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eSGI-DNA for Gibson Assembly® application\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"category-description\"\u003e\n\u003cdiv\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.  \u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003echemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eRoutine cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/p\u003e\n\u003cp\u003eChoice of efficiency:\u003c\/p\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G Chemically Competent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA; (\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e8\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ein 96-well plates).\u003cbr\u003eHighly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G Chemically Competent Cells, Subcloning Grade\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA)\u003cstrong\u003e.  \u003c\/strong\u003e\u003cbr\u003eThe best value available anywhere for simple cloning and plasmid propagation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"195\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"128\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"101\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"216\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cp\u003e10G Chemically Competent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES: without IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cp\u003e10G Chemically Competent Subcloning Grade\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES: without IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eGenotypes\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eF-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003emcrA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eΔ(\u003cem\u003emrr-hsd\u003c\/em\u003eRMS-\u003cem\u003emcr\u003c\/em\u003eBC)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eend\u003c\/em\u003eA1\u003cspan\u003e \u003c\/span\u003e\u003cem\u003erec\u003c\/em\u003eA1 Φ80\u003cem\u003edlac\u003c\/em\u003eZΔM15 Δ\u003cem\u003elac\u003c\/em\u003eX74\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eara\u003c\/em\u003eD139 Δ(\u003cem\u003eara,leu\u003c\/em\u003e)7697\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egal\u003c\/em\u003eU\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egal\u003c\/em\u003eK\u003cspan\u003e \u003c\/span\u003e\u003cem\u003erps\u003c\/em\u003eL\u003cspan\u003e \u003c\/span\u003e\u003cem\u003enup\u003c\/em\u003eG λ-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eton\u003c\/em\u003eA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e 10G Chemically Competent Cells \u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 1 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA;\u003cbr\u003e\u003cstrong\u003e  96-well format\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 1 × 10\u003csup\u003e8\u003c\/sup\u003e in 96-well plates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500317251,"sku":"60107-1","price":876.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953241964611,"sku":"60107-2","price":1246.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_81c0e749-0425-4dad-820c-9423763ddba7.jpg?v=1736396292"},{"product_id":"60108-1","title":"E. cloni-10G Chemically Competent Cells (Subcloning Grade)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G Chemically Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of transformation efficiencies (1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 1 x 10\u003csup\u003e9\u003c\/sup\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency cells result in high colony counts\u003c\/li\u003e\n\u003cli\u003eEndorsed by\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.lucigen.com\/docs\/SGI-App-Note-Featuring-Lucigen-10G-Comp-Cells-High-efficiency-Gibson-Assembly-Constructs.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eSGI-DNA for Gibson Assembly® application\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"category-description\"\u003e\n\u003cdiv\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.  \u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003echemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eRoutine cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/p\u003e\n\u003cp\u003eChoice of efficiency:\u003c\/p\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G Chemically Competent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA; (\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e8\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ein 96-well plates).\u003cbr\u003eHighly efficient competent cells for routine cloning applications.  Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs).  Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G Chemically Competent Cells, Subcloning Grade\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA)\u003cstrong\u003e.  \u003c\/strong\u003e\u003cbr\u003eThe best value available anywhere for simple cloning and plasmid propagation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"195\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"128\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"101\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" width=\"216\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cp\u003e10G Chemically Competent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES: without IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"120\"\u003e\n\u003cp\u003e10G Chemically Competent Subcloning Grade\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e6\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\"\u003e\n\u003cp\u003eYES: without IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eGenotypes\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eF-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003emcrA\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eΔ(\u003cem\u003emrr-hsd\u003c\/em\u003eRMS-\u003cem\u003emcr\u003c\/em\u003eBC)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eend\u003c\/em\u003eA1\u003cspan\u003e \u003c\/span\u003e\u003cem\u003erec\u003c\/em\u003eA1 Φ80\u003cem\u003edlac\u003c\/em\u003eZΔM15 Δ\u003cem\u003elac\u003c\/em\u003eX74\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eara\u003c\/em\u003eD139 Δ(\u003cem\u003eara,leu\u003c\/em\u003e)7697\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egal\u003c\/em\u003eU\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egal\u003c\/em\u003eK\u003cspan\u003e \u003c\/span\u003e\u003cem\u003erps\u003c\/em\u003eL\u003cspan\u003e \u003c\/span\u003e\u003cem\u003enup\u003c\/em\u003eG λ-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eton\u003c\/em\u003eA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e 10G Chemically Competent Cells \u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 1 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA;\u003cbr\u003e\u003cstrong\u003e  96-well format\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 1 × 10\u003csup\u003e8\u003c\/sup\u003e in 96-well plates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"48 rxns","offer_id":18490500448323,"sku":"60108-1","price":670.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_de3ba73d-4476-4dd6-af4a-e3b7a4ee917a.jpg?v=1736396292"},{"product_id":"60110-1","title":"HI-Control-10G Chemically Competent Cells, 12 rxns (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eHI-Control™ BL21(DE3) and HI-Control™ 10G Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003eInduce\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh level protein expression\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efrom\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e based promoters (T7, T5-\u003cem\u003elac\u003c\/em\u003e, tac, trc,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTightly regulate\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eexpression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells\u003c\/li\u003e\n\u003cli\u003eClone targets\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003eand\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ereduce background protein expression with HI-Control 10G Chemically Competent Cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eThe HI-Control™ strains are based on\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cem\u003e\u003cstrong\u003eE. cloni\u003c\/strong\u003e\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003e10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cem\u003e\u003cstrong\u003elac\u003c\/strong\u003e\u003c\/em\u003e\u003cstrong\u003eI repressor gene, which provides tight control over promoters containing the\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cem\u003e\u003cstrong\u003elac\u003c\/strong\u003e\u003c\/em\u003e\u003cstrong\u003eO operator.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReduce leaky expression:\u003c\/strong\u003e The \u003cem\u003eLac\u003c\/em\u003eI repressor protein binds to the \u003cem\u003elac\u003c\/em\u003eO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eObtain high protein yields:\u003c\/strong\u003e Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e-based promoters like T5-\u003cem\u003elac\u003c\/em\u003e, tac, trc, and\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e, can be used in either HI-Control strain.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAchieve high efficiency transformations\u003c\/strong\u003e\u003cstrong\u003e:\u003c\/strong\u003e  Highly efficient competent cells give excellent results from small amounts of plasmid DNA. \u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable width=\" 95%\" border=\"0\" cellpadding=\"2\" class=\"table table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"19%\"\u003e \u003c\/td\u003e\n\u003ctd colspan=\"2\" align=\"center\" valign=\"middle\"\u003e\u003cstrong\u003eTightly Control Protein Expression\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"17%\" align=\"center\" valign=\"middle\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"19%\" align=\"center\" valign=\"middle\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd width=\"22%\" align=\"center\" valign=\"middle\"\u003eT7 Promoters\u003c\/td\u003e\n\u003ctd width=\"23%\" align=\"center\" valign=\"middle\"\u003eNon-T7 lac promoters\u003cbr\u003e(T5-\u003cem\u003elac\u003c\/em\u003e, tac, trc,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e)\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003eClone with low background target expression\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003eTransformation efficiencies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHI-Control\u003cbr\u003eBL21(DE3)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e \u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e≥ 1×10\u003csup\u003e7\u003c\/sup\u003e cfu\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHI-Control\u003cbr\u003e10G\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e \u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e≥ 1×10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eGenotypes:\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eHI-Control™ BL21 (DE3):\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eF-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eompT hsdSB (rB- mB-) gal dcm\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e(DE3) Mini-F\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elacIq1\u003c\/em\u003e(GentR)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHI-Control™ 10G:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003emcrA\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eΔ(\u003cem\u003emrr\u003c\/em\u003e-\u003cem\u003ehsdRMS\u003c\/em\u003e-\u003cem\u003emcrBC\u003c\/em\u003e)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eendA1 recA1\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eɸ80d\u003cem\u003elac\u003c\/em\u003eZΔM15 Δ\u003cem\u003elacX74 araD139\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eΔ (\u003cem\u003eara,leu\u003c\/em\u003e)7697\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egalU galK rpsL\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e(StrR)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003enupG\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eλ−\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etonA\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eMini-F\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elacIq1\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e(GentR)\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500513859,"sku":"60110-1","price":1083.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2e_cd97d8ee-44ed-4f57-a626-928e8f520540.jpg?v=1736396291"},{"product_id":"60117-1","title":"E. cloni-10G CLASSIC Electrocompetent Cells (SixPacks)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® 10G and 10GF' Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)\u003c\/li\u003e\n\u003cli\u003eOptimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies (5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eto 4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg).\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ecompetent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eelectrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs.  These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\n\u003ch2\u003eChoice of strain:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eLibrary construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10GF′Competent Cells.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eContain the F' plasmid for infection with M13 to produce ssDNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eChoice of efficiency:\u003c\/h2\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cspan\u003e \u003c\/span\u003e2\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"hotredbold\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e5\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e×\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e10\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA\u003c\/span\u003e\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid cloning.\u003c\/strong\u003e\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"562\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\" width=\"265\"\u003e\n\u003ch2\u003e\u003cimg alt=\"DH10B Electrocompetent Cells Consistently Outperformed “Ultra High Efficiency” Cells\" border=\"0\" height=\"158\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Recombinant-Yields.gif\" width=\"227\"\u003e\u003c\/h2\u003e\n\u003c\/td\u003e\n\u003ctd width=\"25\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"265\"\u003e\n\u003cp\u003e\u003cimg alt=\"Transformation Efficiency Comparison Between SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen)\" border=\"0\" height=\"150\" src=\"https:\/\/www.lucigen.com\/skin\/common_files\/images\/pages\/Transformation-Efficiency.gif\" width=\"241\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003eTransformation efficiency comparison of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"24 rxns","offer_id":18490500546627,"sku":"60117-1","price":1167.0,"currency_code":"AUD","in_stock":true},{"title":"48 rxns","offer_id":31953326473283,"sku":"60117-2","price":1778.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2a_524e6ad5-a2d0-4616-92b3-537f6e839a56.jpg?v=1736396290"},{"product_id":"60117-2","title":"E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks)","description":"\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for Cloning and Library Construction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"2\" class=\"table table-striped table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"212\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"149\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"82\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\" width=\"60\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\" width=\"91\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e5 × 10\u003csup\u003e9\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eEfficiencies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e 10G SUPREME Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u003c\/strong\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e 2 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg pUC DNA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003cem\u003eE. cloni \u003c\/em\u003e10G CLASSIC Electrocompetent Cells\u003c\/strong\u003e \u003cu\u003e\u0026gt;\u003c\/u\u003e 5 × 10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg pUC DNA \u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Lucigen","offers":[{"title":"EACH","offer_id":18490500579395,"sku":"60117-2","price":1778.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2e_7275dd13-c2d0-4534-92c9-b23b000ae49e.jpg?v=1736396290"},{"product_id":"60210-1","title":"BAC-Optimized Replicator-v2.0 Electrocompetent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eBAC-Optimized Replicator™ v2.0 \u0026amp; 10G BAC-Optimized Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003eCells that enable the most challenging library construction and screening.\u003c\/li\u003e\n\u003cli\u003eHighest available transformation efficiencies for library construction and screening.\u003c\/li\u003e\n\u003cli\u003eOptimized for high yields of DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBAC-Optimized Replicator™ v2.0 Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e(\u0026gt; 1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s\u003cspan\u003e CopyRight®Cloning Kits.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G BAC-Optimized Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg 150 kb BAC DNA;\u003cspan\u003e \u003c\/span\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for BAC or Large Fragment Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"1\" width=\"542\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"182\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"119\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC or pKanR DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"88\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"64\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"88\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003e10G BAC-Optimized Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eBAC-Optimized Replicator v2.0 Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500612163,"sku":"60210-1","price":1186.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953552474179,"sku":"60210-2","price":1764.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2d.jpg?v=1736396289"},{"product_id":"60215-2","title":"E. cloni-10G BAC-Optimized Electrocompetent Cells, 25 rxns (FIVERs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eBAC-Optimized Replicator™ v2.0 \u0026amp; 10G BAC-Optimized Electrocompetent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul type=\"circle\"\u003e\n\u003cli\u003eCells that enable the most challenging library construction and screening.\u003c\/li\u003e\n\u003cli\u003eHighest available transformation efficiencies for library construction and screening.\u003c\/li\u003e\n\u003cli\u003eOptimized for high yields of DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eLucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBAC-Optimized Replicator™ v2.0 Electrocompetent Cells\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e(\u0026gt; 1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eCopyRight\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eCloning Kits\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e10G BAC-Optimized Electrocompetent Cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e(\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg 150 kb BAC DNA;\u003cspan\u003e \u003c\/span\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e \u003c\/span\u003e1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eCompetent Cells for BAC or Large Fragment Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"1\" width=\"542\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"182\"\u003e\n\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eE. cloni\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e\u003c\/strong\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"119\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC or pKanR DNA)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"88\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"64\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\" width=\"88\"\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003e10G BAC-Optimized Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eBAC-Optimized Replicator v2.0 Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd class=\"luc-simple-line-table\"\u003e\n\u003cp align=\"center\"\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"25 rxns","offer_id":18490500677699,"sku":"60215-2","price":1700.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_3bc2539b-82de-47c6-a40e-bb277c90877d.jpg?v=1736396289"},{"product_id":"60240-1","title":"Endura-Chemically Competent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eEndura™ Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eClone repetitive sequences and lentiviral libraries\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStabilize direct repeats and create lentiviral constructs\u003c\/li\u003e\n\u003cli\u003eGenerate\u003cspan\u003e \u003c\/span\u003elentiviral guide RNA libraries\u003c\/li\u003e\n\u003cli\u003eRecommended in\u003cspan\u003e \u003c\/span\u003eCRISPR GeCKO library protocols\u003c\/li\u003e\n\u003cli\u003eChoose electrocompetent or chemically competent cells\u003c\/li\u003e\n\u003cli\u003eHighest efficiency commercially available cells for lentiviral cloning:  over 1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg (chem) or 1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg (electro)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra \u003ca href=\"https:\/\/astralscientific.com.au\/products\/80026-1?variant=18490503659587\"\u003eRecovery Medium (Part No 80026-1)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eConserve clones that contain unstable sequences!\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMaintain unstable DNA.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExcellent value and efficiency!\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSwitch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eChemical or Electrocompetent.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWhichever method of transformation you prefer, Lucigen can give you better efficiency and\/or better prices.\u003c\/p\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eConserve clones that contain unstable sequences!\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMaintain unstable DNA.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExcellent value and efficiency!\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSwitch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eChemical or Electrocompetent.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWhichever method of transformation you prefer, Lucigen can give you better efficiency and\/or better prices.\u003c\/p\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cimg alt=\"Cost per reaction comparison of Endura competent cells and clone-stabilizing strains from other suppliers\" height=\"427\" src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/Endura%20Cells\/Endura-Cost-Rxn.jpg\" width=\"400\"\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eCost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2014\/Endura\/Endura-Efficiency.png\" width=\"359\" height=\"411\" alt=\"Transformation efficiencies of Endura competent cells and other electrocompetent clone-stabilizing strains\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eTransformation efficiencies of electrocompetent clone-stabilizing strains.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500808771,"sku":"60240-1","price":1032.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953459249219,"sku":"60240-2","price":1522.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2a_ee0fe535-e268-451f-a8a5-91e5ea529318.jpg?v=1736396288"},{"product_id":"60241-1","title":"Endura-Chemically Competent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eEndura™ Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eClone repetitive sequences and lentiviral libraries\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStabilize direct repeats and create lentiviral constructs\u003c\/li\u003e\n\u003cli\u003eGenerate\u003cspan\u003e \u003c\/span\u003elentiviral guide RNA libraries\u003c\/li\u003e\n\u003cli\u003eRecommended in\u003cspan\u003e \u003c\/span\u003eCRISPR GeCKO library protocols\u003c\/li\u003e\n\u003cli\u003eChoose electrocompetent or chemically competent cells\u003c\/li\u003e\n\u003cli\u003eHighest efficiency commercially available cells for lentiviral cloning:  over 1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg (chem) or 1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg (electro)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/astralscientific.com.au\/collections\/molecular-biology\/products\/80026-1?_pos=1\u0026amp;_sid=f6f10f5e8\u0026amp;_ss=r\"\u003eRecovery Medium (Part No 80026-1)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eConserve clones that contain unstable sequences!\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMaintain unstable DNA.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExcellent value and efficiency!\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSwitch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eChemical or Electrocompetent.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWhichever method of transformation you prefer, Lucigen can give you better efficiency and\/or better prices.\u003c\/p\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cimg alt=\"Cost per reaction comparison of Endura competent cells and clone-stabilizing strains from other suppliers\" height=\"427\" src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/Endura%20Cells\/Endura-Cost-Rxn.jpg\" width=\"400\"\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eCost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2014\/Endura\/Endura-Efficiency.png\" width=\"359\" height=\"411\" alt=\"Transformation efficiencies of Endura competent cells and other electrocompetent clone-stabilizing strains\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eTransformation efficiencies of electrocompetent clone-stabilizing strains.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500874307,"sku":"60241-1","price":1095.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_4acaa89c-e969-4807-811e-17e978c6c375.jpg?v=1736396287"},{"product_id":"60242-1","title":"Endura-Electrocompetent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eEndura™ Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003ch2\u003eClone repetitive sequences and lentiviral libraries\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStabilize direct repeats and create lentiviral constructs\u003c\/li\u003e\n\u003cli\u003eGenerate\u003cspan\u003e \u003c\/span\u003elentiviral guide RNA libraries\u003c\/li\u003e\n\u003cli\u003eRecommended in\u003cspan\u003e \u003c\/span\u003eCRISPR GeCKO library protocols\u003c\/li\u003e\n\u003cli\u003eChoose electrocompetent or chemically competent cells\u003c\/li\u003e\n\u003cli\u003eHighest efficiency commercially available cells for lentiviral cloning:  over 1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg (chem) or 1 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg (electro)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/astralscientific.com.au\/collections\/molecular-biology\/products\/80026-1?_pos=1\u0026amp;_sid=f6f10f5e8\u0026amp;_ss=r\"\u003eRecovery Medium (Part No 80026-1)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eConserve clones that contain unstable sequences!\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMaintain unstable DNA.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExcellent value and efficiency!\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eSwitch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eChemical or Electrocompetent.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eWhichever method of transformation you prefer, Lucigen can give you better efficiency and\/or better prices.\u003c\/p\u003e\n\u003cdiv class=\"col-md-6\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cimg alt=\"Cost per reaction comparison of Endura competent cells and clone-stabilizing strains from other suppliers\" height=\"427\" src=\"https:\/\/www.lucigen.com\/images\/Products\/2011\/Endura%20Cells\/Endura-Cost-Rxn.jpg\" width=\"400\"\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eCost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-md-6\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2014\/Endura\/Endura-Efficiency.png\" width=\"359\" height=\"411\" alt=\"Transformation efficiencies of Endura competent cells and other electrocompetent clone-stabilizing strains\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eTransformation efficiencies of electrocompetent clone-stabilizing strains.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490500939843,"sku":"60242-1","price":1146.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953463050307,"sku":"60242-2","price":1714.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2f_7c6823ed-794f-45f5-a3b1-952f7580ba5c.jpg?v=1736396287"},{"product_id":"60300-1","title":"E. cloni-EXPRESS BL21(DE3) Electrocompetent Cells","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® EXPRESS BL21(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eBest value for routine protein expression... available as Electrocompetent and Chemically Competent Cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eLucigen’s \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells (Figure 1), which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e EXPRESS Electrocompetent Cells (\u003c\/span\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e 5 × 10\u003c\/span\u003e\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e cfu\/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment (Figure 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"3\" width=\"494\" align=\"center\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"300\"\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2014\/Express\/BL21(DE3)_graph.png\" width=\"500\" height=\"468\" alt=\"BL21(DE3) Efficiency Cell Comparison\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eTransformation efficiency comparison of commercially available BL21(DE3) competent cells.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"542\" align=\"center\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/www.lucigen.com\/images\/products\/EXPRESS_Chart.gif\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of cloning and protein expression procedures.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eEXPRESS BL21(DE3) strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNOTE:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/em\u003eFor best results in expressing proteins that may be toxic, we recommend using\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/astralscientific.com.au\/search?q=OverExpress\u0026amp;type=product\"\u003eOverExpress Cells\u003c\/a\u003e.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eEXPRESS cells are also available as chemically competent cells. With a transformation efficiency of\u003cspan\u003e \u003c\/span\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501005379,"sku":"60300-1","price":884.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31954914967619,"sku":"60300-2","price":1287.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_ea05bf4f-ea24-43d3-8317-b05005b35ed0.jpg?v=1736396286"},{"product_id":"60341-1","title":"OverExpress-C41(DE3) Electrocompetent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501070915,"sku":"60341-1","price":1606.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_17206157-892e-4647-9e7e-71d384c598e9.jpg?v=1736396286"},{"product_id":"60345-1","title":"OverExpress-C43(DE3) Electrocompetent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501136451,"sku":"60345-1","price":1606.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2e_c16c3483-0c63-4bd7-a1c1-72b5a3ae6e87.jpg?v=1736396285"},{"product_id":"60401-1","title":"E. cloni-EXPRESS BL21(DE3)Chemically Competent Cells","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eE. cloni® EXPRESS BL21(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eBest value for routine protein expression... available as Electrocompetent and Chemically Competent Cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eLucigen’s \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells (Figure 1), which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e EXPRESS Electrocompetent Cells (\u003c\/span\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e\u003cspan\u003e 5 × 10\u003c\/span\u003e\u003csup\u003e9\u003c\/sup\u003e\u003cspan\u003e cfu\/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment (Figure 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"3\" width=\"494\" align=\"center\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"300\"\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2014\/Express\/BL21(DE3)_graph.png\" width=\"500\" height=\"468\" alt=\"BL21(DE3) Efficiency Cell Comparison\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eTransformation efficiency comparison of commercially available BL21(DE3) competent cells.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"542\" align=\"center\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/www.lucigen.com\/images\/products\/EXPRESS_Chart.gif\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of cloning and protein expression procedures.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eEXPRESS BL21(DE3) strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eNOTE:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/em\u003eFor best results in expressing proteins that may be toxic, we recommend using\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/astralscientific.com.au\/search?q=OverExpress\u0026amp;type=product\"\u003eOverExpress Cells\u003c\/a\u003e.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eEXPRESS cells are also available as chemically competent cells. With a transformation efficiency of\u003cspan\u003e \u003c\/span\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e1 × 10\u003csup\u003e7\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501201987,"sku":"60401-1","price":693.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31954914050115,"sku":"60401-2","price":831.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_4747bb18-f71b-49db-9663-f7d1879b4401.jpg?v=1736396284"},{"product_id":"60435-1","title":"HI-Control-BL21(DE3) Chemically Competent Cells, 12 rxns (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eHI-Control™ BL21(DE3) and HI-Control™ 10G Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003eInduce\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh level protein expression\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efrom\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e based promoters (T7, T5-\u003cem\u003elac\u003c\/em\u003e, tac, trc,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTightly regulate\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eexpression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells\u003c\/li\u003e\n\u003cli\u003eClone targets\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e\u003cem\u003eand\u003c\/em\u003e\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ereduce background protein expression with HI-Control 10G Chemically Competent Cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eThe HI-Control™ strains are based on\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cem\u003e\u003cstrong\u003eE. cloni\u003c\/strong\u003e\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003e10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cem\u003e\u003cstrong\u003elac\u003c\/strong\u003e\u003c\/em\u003e\u003cstrong\u003eI repressor gene, which provides tight control over promoters containing the\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cem\u003e\u003cstrong\u003elac\u003c\/strong\u003e\u003c\/em\u003e\u003cstrong\u003eO operator.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReduce leaky expression:\u003c\/strong\u003e The \u003cem\u003eLac\u003c\/em\u003eI repressor protein binds to the \u003cem\u003elac\u003c\/em\u003eO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eObtain high protein yields:\u003c\/strong\u003e Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e-based promoters like T5-\u003cem\u003elac\u003c\/em\u003e, tac, trc, and\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e, can be used in either HI-Control strain.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAchieve high efficiency transformations\u003c\/strong\u003e\u003cstrong\u003e:\u003c\/strong\u003e  Highly efficient competent cells give excellent results from small amounts of plasmid DNA. \u003c\/p\u003e\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable width=\" 95%\" border=\"0\" cellpadding=\"2\" class=\"table table-bordered\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"19%\"\u003e \u003c\/td\u003e\n\u003ctd colspan=\"2\" align=\"center\" valign=\"middle\"\u003e\u003cstrong\u003eTightly Control Protein Expression\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"17%\" align=\"center\" valign=\"middle\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"19%\" align=\"center\" valign=\"middle\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd width=\"22%\" align=\"center\" valign=\"middle\"\u003eT7 Promoters\u003c\/td\u003e\n\u003ctd width=\"23%\" align=\"center\" valign=\"middle\"\u003eNon-T7 lac promoters\u003cbr\u003e(T5-\u003cem\u003elac\u003c\/em\u003e, tac, trc,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003e)\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003eClone with low background target expression\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003eTransformation efficiencies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHI-Control\u003cbr\u003eBL21(DE3)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e \u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e≥ 1×10\u003csup\u003e7\u003c\/sup\u003e cfu\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHI-Control\u003cbr\u003e10G\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e \u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e\u003cspan class=\"glyphicon glyphicon-ok\"\u003e\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd align=\"center\" valign=\"middle\"\u003e≥ 1×10\u003csup\u003e9\u003c\/sup\u003e cfu\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eGenotypes:\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eHI-Control™ BL21 (DE3):\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eF-\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eompT hsdSB (rB- mB-) gal dcm\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e(DE3) Mini-F\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elacIq1\u003c\/em\u003e(GentR)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHI-Control™ 10G:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003emcrA\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eΔ(\u003cem\u003emrr\u003c\/em\u003e-\u003cem\u003ehsdRMS\u003c\/em\u003e-\u003cem\u003emcrBC\u003c\/em\u003e)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eendA1 recA1\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eɸ80d\u003cem\u003elac\u003c\/em\u003eZΔM15 Δ\u003cem\u003elacX74 araD139\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eΔ (\u003cem\u003eara,leu\u003c\/em\u003e)7697\u003cspan\u003e \u003c\/span\u003e\u003cem\u003egalU galK rpsL\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e(StrR)\u003cspan\u003e \u003c\/span\u003e\u003cem\u003enupG\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eλ−\u003cspan\u003e \u003c\/span\u003e\u003cem\u003etonA\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eMini-F\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elacIq1\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003e(GentR)\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501300291,"sku":"60435-1","price":1083.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_fd370bfd-60f5-461c-a093-ee7d558bcad6.jpg?v=1736396284"},{"product_id":"60442-1","title":"OverExpress-C41(DE3) Chemically Competent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501333059,"sku":"60442-1","price":1398.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_a7f0e972-019a-4179-99d2-e5d2b48ed386.jpg?v=1736396283"},{"product_id":"60444-1","title":"OverExpress-C41(DE3) pLysS Chemically Competent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501398595,"sku":"60444-1","price":1398.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2d_ee6b20c5-68c4-41fb-965f-4ead5c239828.jpg?v=1736396283"},{"product_id":"60446-1","title":"OverExpress-C43(DE3) Chemically Competent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501464131,"sku":"60446-1","price":1398.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2a_10352b82-1bdb-4ca3-8fcd-eaa7d0f6fe7f.jpg?v=1736396282"},{"product_id":"60448-1","title":"OverExpress-C43(DE3) pLysS Chemically Competent Cells (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490501529667,"sku":"60448-1","price":1398.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_c9349fc1-a261-4b5a-8d7d-85fbae0bbac6.jpg?v=1736396281"},{"product_id":"60452-1","title":"OverExpress-ChemComboPack, 12 rxns (SOLOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003eOverExpress™ C41(DE3) and C43(DE3) Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic \u0026amp; membrane proteins\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eBL21(DE3) strains, like Lucigen’s\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. cloni\u003c\/em\u003e® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\n\u003cp\u003eLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003estrains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been\u003cspan\u003e \u003c\/span\u003evalidated in more than 350 publications.\u003c\/p\u003e\n\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"391\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"230\"\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/Protein-Express.png\" width=\"261\" height=\"400\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eGreen Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eTable 1 and Figure 2 summarize transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eTable 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003ctable cellpadding=\"5\" class=\"table table-bordered table-responsive\"\u003e\n\u003ctbody\u003e\n\u003ctr bgcolor=\"#cccccc\" valign=\"middle\"\u003e\n\u003ctd width=\"65\" height=\"31\" valign=\"bottom\"\u003e\u003cstrong\u003eStrain\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"141\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eTransformation\u003cbr\u003eSuccess Rate\u003csup\u003ea\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"190\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpression-induced Toxicity\u003csup\u003eb\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"151\" align=\"center\" valign=\"bottom\"\u003e\n\u003cdiv align=\"center\"\u003e\u003cstrong\u003eExpressing Plasmids\u003csup\u003ec\u003c\/sup\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eBL21(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e16\/26 (62%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e25\/26 (96%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/26 (54%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC41(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e14\/28 (50%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e24\/28 (86%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003eC43(DE3)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e28\/28 (100%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e1\/28 (4%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd bordercolor=\"#333333\"\u003e\n\u003cdiv align=\"center\"\u003e23\/28 (81%)\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003ctable align=\"center\" border=\"0\" cellpadding=\"0\" width=\"400\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/OverExpress\/OverExpress-Comparison.png\" width=\"550\" height=\"295\" alt=\"\"\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp class=\"figure\"\u003e\u003cstrong\u003eFigure 2.\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eComparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"figure\"\u003e\u003csup\u003ea\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eTransformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003eb\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003e\u003csup\u003ec\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eExpressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004).\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eProtein Expression and Purification\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e37\u003c\/strong\u003e, 203-206. Data used with permission.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elac\u003c\/em\u003eUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003elon and ompT\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eproteases.\u003c\/p\u003e\n\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.\u003c\/p\u003e\n\u003ch3\u003eFAQ Which OverExpress cell strain should I use?\u003c\/h3\u003e\n\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\n\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490502381635,"sku":"60452-1","price":1398.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2d_b1c41209-4bf1-4adb-adf4-ff0c37a855c3.jpg?v=1736396281"},{"product_id":"60502-1","title":"TG1 Electrocompetent Cells (DUOs)","description":"\u003cdiv class=\"d_underlined\"\u003e\n\u003ch1 class=\"dialog_title\"\u003ePhage Display Competent Cells\u003c\/h1\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"subcategory-descr\"\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003ePerfect for antibody phage display or peptide phage display library creation\u003c\/li\u003e\n\u003cli\u003eThe highest efficiency TG1 competent cells available: ≥4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg.\u003c\/li\u003e\n\u003cli\u003eYour only source for electrocompetent SS320 and ER2738 cells\u003c\/li\u003e\n\u003cli\u003eAmber suppressor and non-amber suppressor lines for maximum flexibility\u003c\/li\u003e\n\u003cli\u003eHigh efficiency\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eMC1061 F- cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor cloning or control reactions\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eMC1061 F- Electrocompetent Cells.\u003c\/strong\u003e\u003cspan\u003e A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 10\u003c\/span\u003e\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e cfu\/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage.  \u003c\/span\u003e\u003cem\u003eThis strain not included in the sample kit.\u003c\/em\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eTG1 Electrocompetent Cells.\u003c\/strong\u003e An amber suppressor strain (\u003cem\u003esup\u003c\/em\u003eE) prepared as highly efficient (≥4 × 10\u003csup\u003e10\u003c\/sup\u003e cfu\/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eSS320 Electrocompetent Cells.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eA non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.\u003c\/li\u003e\n\u003cli\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eER2738 Electrocompetent Cells.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eAn amber suppressor strain (\u003cem\u003egln\u003c\/em\u003eV) prepared as highly efficient (≥2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecfu\/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"center\"\u003e\n\u003ctable border=\"0\" cellpadding=\"10\" width=\"450 px\" class=\"table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/www.lucigen.com\/images\/Products\/2015\/Phage-Display\/Phage-Display-Tranformation-Efficiency.png\" width=\"500\" height=\"351\" alt=\"Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitors\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr width=\"500\"\u003e\n\u003ctd width=\"500\" align=\"left\"\u003e\n\u003cstrong\u003eFigure 1.\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eTransformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"5\" cellspacing=\"3\" width=\"542\" class=\"table table-responsive table-striped table-bordered\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003ctd width=\"176\" valign=\"bottom\"\u003e\n\u003cbr\u003e\u003cstrong\u003eCell Lines\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd width=\"108\" align=\"center\" valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation Efficiency\u003c\/strong\u003e\u003cbr\u003e\u003cspan class=\"figure\"\u003e(cfu\/µg pUC DNA)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"82\" align=\"center\" valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eCloning Methylated DNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"60\" align=\"center\" valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eBAC, Cosmid Cloning\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"116\" align=\"center\" valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4× 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eMC1061 F- Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e4× 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003e\u003cu\u003e\u0026gt;\u003c\/u\u003e2 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd align=\"center\" bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eNO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd bgcolor=\"#FFFFFF\"\u003e\n\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"Lucigen","offers":[{"title":"12 rxns","offer_id":18490503069763,"sku":"60502-1","price":1170.0,"currency_code":"AUD","in_stock":true},{"title":"24 rxns","offer_id":31953413472323,"sku":"60502-2","price":1746.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0046\/6599\/9427\/products\/Competent_Cells_Range2c_c1632f0b-4c26-4d4e-87d6-d4d78321f8af.jpg?v=1736396280"}],"url":"https:\/\/astralscientific.com.au\/collections\/300-dry-ice-fee.oembed?page=5","provider":"Astral Scientific","version":"1.0","type":"link"}