T4 DNA Ligase catalyzes joins strands of dsRNA and dsDNA by forming phosphodiester bonds. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

10X T4 DNA Ligase Buffer is composed of 500mM Tris-HCI, 100 mM MgCl₂, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25°C. 2X Rapid Ligation Buffer is composed of 132mM Tris-HCI, 20 mM MgCl₂, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25°C.

Specifications

Unit Definition: 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1 X T4 DNA Ligase Buffer following a 30 minute incubation at 23°C.

Source: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.