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NxSeq® AmpFREE Low DNA Library Kits & Adaptors (Illumina-compatible)

The highest efficiency NGS fragment library prep kit available at the lowest cost

  • Great Performance: High efficiency libraries mean more sequenceable fragments in each library, better coverage and depth from single or multiplexed libraries which improves confidence in each sequencing run.
  • Minimal Bias: PCR-free DNA libraries produce more uniform coverage from as little as 75 ng of sheared input DNA.
  • Fast: 2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.
  • Affordable: Best priced and best performing kit available.
  • Special Offer: Save 50% on the 12 rxn kit through 29th April 2016.

You're doing next gen sequencing for a reason; you want big data! To get there, you know you need the best fragment libraries possible to get the most information out of each sample. So why spend your time, resources and expensive sequencing reagents on anything but the best fragment DNA libraries?

The NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimised each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours using a streamlined, easy to follow protocol.

  • Better Data
  • Higher efficiency libraries
  • More clusters identified
  • Excellent sequencing data examples
  • Minimal GC bias
  • Lower price
  • Faster, easier protocol

Higher Efficiency Libraries

More Sequenceable DNA Fragments per Library = More Data

NxSeq Fragment Library Kit Most Efficient

Figure 1. Percentage of library DNA with correctly ligated adaptors measured by qPCR. Duplicate libraries were prepped per kit/organism (Human, Staphylococcus aureus, Rhodobacter sphaeroides (1 library only), and E. coli) according to the manufacturer’s recommended input amounts and protocols. Adaptor ligation efficiency was measured by qPCR using the KAPA Library Quantification Kit and matching amplified library as an internal standard.

Greater Percentage of Identifiable Clusters

More DNA Fragments Sequenced per Run = More Data

NxSeq Fragment Library Kit Most DNA Fragments Sequenced

Figure 2. Percentage of clusters identified upon sequencing various library prep kits. Prepped each library per kit/organism (Human, Staphylococcus aureus, Rhodobacter sphaeroides, and E. coli) according to the manufacturer’s recommended input amounts and protocols. Quantitated and normalized samples to 2 nM final concentrations using the Bioanalyzer and Qubit fluorometer. Ran 5 µL of each sample on a MiSeq using 2 x 150 bp chemistry.

 

Highly Mappable Reads (>92%) from Human, Staphylococcus and Rhodobacter gDNA Sequencing

Sequencing Stat

Human Staphylococcus

Rhodobacter

Genome size, GC percentage

~3 Gbp  45% GC

2,821,361  33% GC

4,602,977  69% GC

Raw reads

3,131,114

1,260,836

3,900,174

Mapped reads

2,979,237 (95.15%)

1,174,111 (93.12%)

3,613,165 (92.64%)

Read length

148.9 bp

148.8 bp

149.6 bp

Total bases

443,767,447

174,694,261

540,403,552

Genome fraction

0.11

0.97

1.00

Avg. coverage

0.15X

62X

117X

Figure 3. Representative gDNA sequencing stats from three different organisms. Genomic DNA fragment libraries were generated using the NxSeq AmpFREE Low DNA Library Kit using 75 ng of sheared gDNA input from three organisms (human, Staphylococcus aureus, and Rhodobacter sphaeroides). The final libraries were quantitated and normalized to 2 nM final concentrations using the Bioanalyzer and Qubit fluorometer, and 5 µL of each library was run on a MiSeq using 2 x 150 bp chemistry and analyzed.

 

Minimal Bias Detected

NxSeq Fragment Library Kit Minimal Sequencing Bias
Figure 4. Sequencing bias measured for three different organisms with varying GC content. DNA fragment libraries were generated from gDNA of three organisms with varying GC content (Staphylococcus aureus, 24% GC; E. coli K12, 50% GC; and Rhodobacter sphaeroides, 68% GC) according to the manufacturer’s recommended input amounts and protocols. Samples were quantitated using the Bioanalyzer and Qubit fluorometer  and normalized to 2 nM final concentrations. Five µL of each library sample was sequenced on a MiSeq using 2 x 150 bp v2 chemistry and analyzed. Normalized coverage was calculated as the (average coverage of all windows with X% GC content) divided by the (overall average coverage).

 

Don’t spend 25-74% More per Library with Another Kit

Save Big with the NxSeq® AmpFREE Low DNA Library Kit

PCR-Free Fragment Library Kit Cat. No. Size
(rxns)
2016 US
List Price
Cost/Reaction Per Rxn Cost Increase
vs. NxSeq® Kit
Lucigen NxSeq®
AmpFREE Low
DNA Library Kit
14000-1 12 -- --
14000-2 48 -- -- 12 rxn 48 rxn
Kapa Hyper Prep Kit KK8501 8 -- -- +65% +74%
KK8503 24 -- -- +45% +53%
KK8505 96 -- -- +25% +32%

 

Faster Protocol with Significantly Less Hands-on Time

Save Time and Get Your Samples on the Sequencer Sooner!

NxSeq Fragment Library Kit Fastest Protocol

 

Ordering Information

The 12 reaction kit is available at 50% off until 29th April 2016 using the promotion Part No. 14000-1P. After this date only Part No. 14000-1 will be available.

The NxSeq Library Kit and Adaptors are only compatible with Illumina sequencers. Each NxSeq Library Kit contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Each box of NxSeq Adaptors contains (12) different indexed Illumina compatible adaptors with enough of each adapter for 4 reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.

Storage and Shipping

All products listed are stored at (-10°C) to (-30°C) and shipped on dry ice.

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Product Code Product Name QTY Price Action
14000-2 NxSeq® AmpFREE Low DNA Library Kits 48 rxns
$1,830.00
14000-1 NxSeq® AmpFREE Low DNA Library Kits 12 rxns
$495.00
14400-1 NxSeq® Adaptors, Box 2 - 12 x 4 rxns
$319.00
14300-1 NxSeq® Adaptors, Box 1 - 12 x 4 rxns
$319.00

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