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BigEasy®-TSA™ Electrocompetent Cells

  • Cells required for transformation and propagation of pJAZZ® Vectors.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimised for high yields of DNA.

BigEasy™-TSA™ Electrocompetent Cells (>2 × 1010 cfu/µg pKanR DNA) are for use with Lucigen’s pJAZZ®-OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System. The TSA Cells have the telN gene required for high efficiency transformation, and the antA gene required for copy number control with the pJAZZ vector, integrated into the host genome. These cells also give high yield and high quality plasmid DNA due to the endA1 and recA1 mutations. They contain the mcr and mrr mutations, allowing methylated genomic DNA that has been isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.

Table 1. E. cloni Competent Cells for BAC, Large or Difficult Fragment Cloning

E. cloniCell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

BigEasy-TSA Electrocompetent

>2 × 1010



IPTG induction required

BigEasy-TSA: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1araD139 Δ(ara,leu)7697 galgalrpsnupG λ- tonAmpR sopAB telantA


Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

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Product Code Product Name QTY Price Action
60224-2 BigEasy TSA ElectroComp.Cells SOLOS 12rxns
60224-3 BigEasy TSA ElectroComp.Cells SOLOS 24rxns
60224-1 BigEasy TSA ElectroComp.Cells SOLOS 6rxn

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