Astral Scientific is one of Australia’s Leading Supplier for Life Science, Diagnostic and Compounding Pharmacy Supplies.

Call us on: (02) 9540 2055

You have no items in your shopping cart.

Subscribe to RSS Feed

FailSafe™ PCR Systems

Dependable, consistent high-fidelity PCR results with every target DNA template.

  • Successful PCR, the first time and every time
  • PCR amplification of difficult or high-GC templates (Fig. 1)
  • Multiplex PCR compatible (Fig. 2)
  • Robust amplification of targets up to 20 kb long
  • High accuracy with 3-fold lower error rate than Taq DNA polymerase (error rate = 1 in 30,000)
  • Extremely high sensitivity and specificity using the PCR Enhancer Technology (with Betaine)
 

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe™ PCR System will faithfully amplify your template every time….We promise!

Applications

  • End-point PCR optimization
  • Consistent and reproducible PCR

How the FailSafe System Works

  1. First, purchase the FailSafe PCR PreMix Selection Kit (catalog number FS99060). The FailSafe PCR PreMix Selection Kit is composed of:
    • FailSafe PCR Enzyme Blend (60 Units)
    • Twelve (12) different FailSafe™ 2X PCR PreMixes. Each 2X PreMix contains dNTPs, Buffer and different concentrations of Mg+2 and Epicentre's proprietary PCR Enhancer (with Betaine).
  2. Prepare and perform PCR reactions using your template and PCR primers with each of the 12 FailSafe™ 2X PCR PreMixes and the FailSafe PCR Enzyme.
  3. Analyze, by agarose gel electrophoresis, the PCR product produced from each of the 12 reactions and visually determine which PreMix gives the best amplification (e.g., a single, strong PCR product of the desired length or "as expected" multiplex PCR results). We guarantee that one of the FailSafe PCR 2X PreMixes will give the desired amplification product.
  4. For reliable and consistent PCR results, use the FailSafe PCR Enzyme and the best 2X PreMix indentified in the optimization experiments for all subsequent amplifications with that template/primer combination.
  5. To reduce costs, purchase a FailSafe PCR System (Cat. No. FS99100, FS99250,or FS9901K) which provides sufficient enzyme and the appropriate amount of your favorite 2X PCR Premix(es) instead of buying the enzyme and 2X PCR Premixes separately.
 
Figure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe™ PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow. Figure 2. Multiplex PCR of the human CFTR gene. The FailSafe™ PCR System amplified all five exons of the CFTR gene from as little as 1 ng of human genomic DNA. L

List  Grid 

16 Item(s)

Set Descending Direction
per page
Product Code Product Name QTY Price Action
FSP995A FailSafe™ PCR 2X PreMix A 2.5ml
$101.00
FSP995B FailSafe™ PCR 2X PreMix B 2.5ml
$101.00
FSP995C FailSafe™ PCR 2X PreMix C 2.5ml
$101.00
FSP995D FailSafe™ PCR 2X PreMix D 2.5ml
$101.00
FSP995E FailSafe™ PCR 2X PreMix E 2.5ml
$101.00
FSP995F FailSafe™ PCR 2X PreMix F 2.5ml
$101.00
FSP995G FailSafe™ PCR 2X PreMix G 2.5ml
$101.00
FSP995H FailSafe™ PCR 2X PreMix H 2.5ml
$101.00
FSP995I FailSafe™ PCR 2X PreMix I 2.5ml
$101.00
FSP995J FailSafe™ PCR 2X PreMix J 2.5ml
$101.00
FSP995K FailSafe™ PCR 2X PreMix K 2.5ml
$101.00
FSP995L FailSafe™ PCR 2X PreMix L 2.5ml
$101.00
FS99060 FailSafe™ PCR PreMix Selection Kit
$213.00
FS9901K FailSafe™ PCR System 1,000 U
$2,157.00
FS99100 FailSafe™ PCR System 100 U
$263.00
FS99250 FailSafe™ PCR System 250 U
$610.00

List  Grid 

16 Item(s)

Set Descending Direction
per page