The BigEasy Kit with the pJAZZ vector is ideal for constructing bias-free, large insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb.  Because the novel pJAZZ vector is linear, the ends of the vector can rotate freely.  As a result, the vector does not supercoil.  Without the torsional stress induced by supercoiling, otherwise unclonable sequences (e.g., repetitive, or A/T- or G/C-rich sequences) are stabilized.  The pJAZZ linear cloning vector is maintained at low copy number (5-10/cell) in BigEasy-TSA™ Electrocompetent Cells (>4 x 10¹⁰ cfu/µg), which are required for transformation and propagation. The copy number can be induced 5-10X in this strain.  In addition, the pJAZZ vector incorporates Lucigen’s CloneSmart® technology for transcription-free cloning, which further increases insert stability.  The pJAZZ vector can be isolated using standard plasmid prep methods.

Convenient Success

Efficiently obtain your clone or create your library

The BigEasy v2.0 Kits eliminate tedious vector and competent cell preparation, as well as time-consuming QC testing experiments.  Kits include optimized reagents, ligation-ready vector, highly efficient electrocompetent cells (not applicable to fosmid kits), detailed instructions, and trouble-shooting guides to simplify cloning and sequencing.